CML10, a variant of calmodulin, modulates ascorbic acid synthesis

Kwang Moon Cho, Ha Thi Kim Nguyen, Soo Youn Kim, Jin Seok Shin, Dong Hwa Cho, Seung Beom Hong, Jeong Sheop Shin, Sung Han Ok

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Calmodulins (CaMs) regulate numerous Ca2+-mediated cellular processes in plants by interacting with their respective downstream effectors. Due to the limited number of CaMs, other calcium sensors modulate the regulation of Ca2+-mediated cellular processes that are not managed by CaMs. Of 50 CaM-like (CML) proteins identified in Arabidopsis thaliana, we characterized the function of CML10. Yeast two-hybrid screening revealed phosphomannomutase (PMM) as a putative interaction partner of CML10. In vitro and in vivo interaction assays were performed to analyze the interaction mechanisms of CML10 and PMM. PMM activity and the phenotypes of cml10 knock-down mutants were studied to elucidate the role(s) of the CML10-PMM interaction. PMM interacted specifically with CML10 in the presence of Ca2+ through its multiple interaction motifs. This interaction promoted the activity of PMM. The phenotypes of cml10 knock-down mutants were more sensitive to stress conditions than wild-type plants, corresponding with the fact that PMM is an enzyme which modulates the biosynthesis of ascorbic acid, an antioxidant. The results of this research demonstrate that a calcium sensor, CML10, which is an evolutionary variant of CaM, modulates the stress responses in Arabidopsis by regulating ascorbic acid production.

Original languageEnglish
Pages (from-to)664-678
Number of pages15
JournalNew Phytologist
Volume209
Issue number2
DOIs
Publication statusPublished - 2016 Jan 1

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Keywords

  • Arabidopsis thaliana
  • Abiotic stress
  • Ascorbic acid (AsA)
  • Calcium signaling
  • Calmodulin (CaM)
  • Calmodulin-like (CML)
  • Phosphomannomutase (PMM)

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Cho, K. M., Nguyen, H. T. K., Kim, S. Y., Shin, J. S., Cho, D. H., Hong, S. B., Shin, J. S., & Ok, S. H. (2016). CML10, a variant of calmodulin, modulates ascorbic acid synthesis. New Phytologist, 209(2), 664-678. https://doi.org/10.1111/nph.13612