Co-Culture model using THP-1 cell and HUVEC on AGEs-Induced expression of cytokines and RAGE

Kwang Won Lee, Hyun Sun Lee

Research output: Contribution to journalArticlepeer-review


Although monoculture methods have been remarkably useful due to their simplicity, they have serious limitation because of the different types of cells communication with each other in many physiological situations. We demonstrated levels of markers of endothelial dysfunction such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as stimulation of receptor of advanced glycation endproducts (AGEs) on monoand co-culture system such as only monocyte (THP-1) cultivation system, only endothelial cell (HUVEC) cultivation system, and co-cultivation system of THP-1 and HUVEC. The mRNA levels of TNF-α and IL-1β on HUVEC increased by the co-culture with monocyte after 4 hr at 100 μg/mL glyceraldehyde-AGE. The secreted protein contents into medium of TNF-α and IL-1β increased after 8 hr approximately 2~2.5 times compared to mono-cultivation. In contrast, the mRNA level of receptor of AGE (RAGE) was relatively insensitive on the co-culture system. The mediators by which monocytes activate endothelial cell have not been fully elucidated. In this study we confirmed production of soluble cytokines such as TNF-α and IL-1β by monocytes. Use of monocyte conditioned medium, which contains both cytokines, can activate endothelial cell.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalJournal of the Korean Society of Food Science and Nutrition
Issue number3
Publication statusPublished - 2011


  • Advanced glycation endproducts (AGEs)
  • Co-culture
  • Endothelial cell
  • Endothelial dysfunction
  • Monocyte

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


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