Co-Culture model using THP-1 cell and HUVEC on AGEs-Induced expression of cytokines and RAGE

Kwang Won Lee, Hyun Sun Lee

Research output: Contribution to journalArticle

Abstract

Although monoculture methods have been remarkably useful due to their simplicity, they have serious limitation because of the different types of cells communication with each other in many physiological situations. We demonstrated levels of markers of endothelial dysfunction such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as stimulation of receptor of advanced glycation endproducts (AGEs) on monoand co-culture system such as only monocyte (THP-1) cultivation system, only endothelial cell (HUVEC) cultivation system, and co-cultivation system of THP-1 and HUVEC. The mRNA levels of TNF-α and IL-1β on HUVEC increased by the co-culture with monocyte after 4 hr at 100 μg/mL glyceraldehyde-AGE. The secreted protein contents into medium of TNF-α and IL-1β increased after 8 hr approximately 2~2.5 times compared to mono-cultivation. In contrast, the mRNA level of receptor of AGE (RAGE) was relatively insensitive on the co-culture system. The mediators by which monocytes activate endothelial cell have not been fully elucidated. In this study we confirmed production of soluble cytokines such as TNF-α and IL-1β by monocytes. Use of monocyte conditioned medium, which contains both cytokines, can activate endothelial cell.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalJournal of the Korean Society of Food Science and Nutrition
Volume40
Issue number3
DOIs
Publication statusPublished - 2011 Sep 7

Fingerprint

Cytokine Receptors
Human Umbilical Vein Endothelial Cells
coculture
Coculture Techniques
monocytes
tumor necrosis factors
interleukin-1
Monocytes
cytokines
Interleukin-1
Tumor Necrosis Factor-alpha
receptors
endothelial cells
Endothelial Cells
cells
Cytokines
glyceraldehyde
Glyceraldehyde
cell communication
Messenger RNA

Keywords

  • Advanced glycation endproducts (AGEs)
  • Co-culture
  • Endothelial cell
  • Endothelial dysfunction
  • Monocyte

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Co-Culture model using THP-1 cell and HUVEC on AGEs-Induced expression of cytokines and RAGE. / Lee, Kwang Won; Lee, Hyun Sun.

In: Journal of the Korean Society of Food Science and Nutrition, Vol. 40, No. 3, 07.09.2011, p. 385-392.

Research output: Contribution to journalArticle

@article{50bcdade8a2c4393ab041b30cf096b1f,
title = "Co-Culture model using THP-1 cell and HUVEC on AGEs-Induced expression of cytokines and RAGE",
abstract = "Although monoculture methods have been remarkably useful due to their simplicity, they have serious limitation because of the different types of cells communication with each other in many physiological situations. We demonstrated levels of markers of endothelial dysfunction such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as stimulation of receptor of advanced glycation endproducts (AGEs) on monoand co-culture system such as only monocyte (THP-1) cultivation system, only endothelial cell (HUVEC) cultivation system, and co-cultivation system of THP-1 and HUVEC. The mRNA levels of TNF-α and IL-1β on HUVEC increased by the co-culture with monocyte after 4 hr at 100 μg/mL glyceraldehyde-AGE. The secreted protein contents into medium of TNF-α and IL-1β increased after 8 hr approximately 2~2.5 times compared to mono-cultivation. In contrast, the mRNA level of receptor of AGE (RAGE) was relatively insensitive on the co-culture system. The mediators by which monocytes activate endothelial cell have not been fully elucidated. In this study we confirmed production of soluble cytokines such as TNF-α and IL-1β by monocytes. Use of monocyte conditioned medium, which contains both cytokines, can activate endothelial cell.",
keywords = "Advanced glycation endproducts (AGEs), Co-culture, Endothelial cell, Endothelial dysfunction, Monocyte",
author = "Lee, {Kwang Won} and Lee, {Hyun Sun}",
year = "2011",
month = "9",
day = "7",
doi = "10.3746/jkfn.2011.40.3.385",
language = "English",
volume = "40",
pages = "385--392",
journal = "Journal of the Korean Society of Food Science and Nutrition",
issn = "1226-3311",
publisher = "Korean Society of Food Science and Nutrition",
number = "3",

}

TY - JOUR

T1 - Co-Culture model using THP-1 cell and HUVEC on AGEs-Induced expression of cytokines and RAGE

AU - Lee, Kwang Won

AU - Lee, Hyun Sun

PY - 2011/9/7

Y1 - 2011/9/7

N2 - Although monoculture methods have been remarkably useful due to their simplicity, they have serious limitation because of the different types of cells communication with each other in many physiological situations. We demonstrated levels of markers of endothelial dysfunction such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as stimulation of receptor of advanced glycation endproducts (AGEs) on monoand co-culture system such as only monocyte (THP-1) cultivation system, only endothelial cell (HUVEC) cultivation system, and co-cultivation system of THP-1 and HUVEC. The mRNA levels of TNF-α and IL-1β on HUVEC increased by the co-culture with monocyte after 4 hr at 100 μg/mL glyceraldehyde-AGE. The secreted protein contents into medium of TNF-α and IL-1β increased after 8 hr approximately 2~2.5 times compared to mono-cultivation. In contrast, the mRNA level of receptor of AGE (RAGE) was relatively insensitive on the co-culture system. The mediators by which monocytes activate endothelial cell have not been fully elucidated. In this study we confirmed production of soluble cytokines such as TNF-α and IL-1β by monocytes. Use of monocyte conditioned medium, which contains both cytokines, can activate endothelial cell.

AB - Although monoculture methods have been remarkably useful due to their simplicity, they have serious limitation because of the different types of cells communication with each other in many physiological situations. We demonstrated levels of markers of endothelial dysfunction such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) as well as stimulation of receptor of advanced glycation endproducts (AGEs) on monoand co-culture system such as only monocyte (THP-1) cultivation system, only endothelial cell (HUVEC) cultivation system, and co-cultivation system of THP-1 and HUVEC. The mRNA levels of TNF-α and IL-1β on HUVEC increased by the co-culture with monocyte after 4 hr at 100 μg/mL glyceraldehyde-AGE. The secreted protein contents into medium of TNF-α and IL-1β increased after 8 hr approximately 2~2.5 times compared to mono-cultivation. In contrast, the mRNA level of receptor of AGE (RAGE) was relatively insensitive on the co-culture system. The mediators by which monocytes activate endothelial cell have not been fully elucidated. In this study we confirmed production of soluble cytokines such as TNF-α and IL-1β by monocytes. Use of monocyte conditioned medium, which contains both cytokines, can activate endothelial cell.

KW - Advanced glycation endproducts (AGEs)

KW - Co-culture

KW - Endothelial cell

KW - Endothelial dysfunction

KW - Monocyte

UR - http://www.scopus.com/inward/record.url?scp=80052318098&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052318098&partnerID=8YFLogxK

U2 - 10.3746/jkfn.2011.40.3.385

DO - 10.3746/jkfn.2011.40.3.385

M3 - Article

AN - SCOPUS:80052318098

VL - 40

SP - 385

EP - 392

JO - Journal of the Korean Society of Food Science and Nutrition

JF - Journal of the Korean Society of Food Science and Nutrition

SN - 1226-3311

IS - 3

ER -