TY - JOUR
T1 - Co-culture of human bone marrow mesenchymal stem cells and macrophages attenuates lipopolysaccharide-induced inflammation in human corneal epithelial cells
AU - Jeong, Won Yong
AU - Kim, Ji Hye
AU - Kim, Chan Wha
N1 - Funding Information:
This work was supported by Korea University, with additional support from Korea University BK21 Plus Program (School of Life Sciences and Biotechnology, Korea University).
Publisher Copyright:
© 2018 Japan society for Bioscience, Biotechnology, and agrochemistry.
PY - 2018
Y1 - 2018
N2 - Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group. Moreover, the IL-1 Receptor Antagonist (IL-1RA) was highly expressed in the CM from the HBMSCs + macrophages group. Wounded eyes of mice were treated with IL-1RA at 0–100 ng/mL for 16 h, the wound size was reduced. The results of this study might lead to the identification of new therapeutic targets for DES.
AB - Dry eye syndrome (DES) is considered as an ocular surface inflammatory disease. Previous studies have shown inflammation plays an important role in the progression and onset of DES. Co-culture of human bone marrow mesenchymal stem cells (HBMSCs) and macrophages showed immunomodulatory effects via regulation of cytokine regulation. Thus, the aim of this study was to investigate the effect of the interaction of these cells on in vitro DES model. The conditioned media (CM) from macrophages, HBMSCs, and HBMSCs + macrophages were treated to human corneal epithelial cells, which showed significant reduction in IL-1α and IL-1β expression levels in HBMSCs + macrophages group. Moreover, the IL-1 Receptor Antagonist (IL-1RA) was highly expressed in the CM from the HBMSCs + macrophages group. Wounded eyes of mice were treated with IL-1RA at 0–100 ng/mL for 16 h, the wound size was reduced. The results of this study might lead to the identification of new therapeutic targets for DES.
KW - Dry eye syndrome
KW - Human corneal epithelial cells
KW - Il-1 receptor antagonist
KW - Mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=85046693359&partnerID=8YFLogxK
U2 - 10.1080/09168451.2018.1438167
DO - 10.1080/09168451.2018.1438167
M3 - Article
C2 - 29452534
AN - SCOPUS:85046693359
SN - 0916-8451
VL - 82
SP - 800
EP - 809
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 5
ER -
