Co-culture of tumor spheroids and fibroblasts in a collagen matrix-incorporated microfluidic chip mimics reciprocal activation in solid tumor microenvironment

Su Yeong Jeong, Ji Hyun Lee, Yoojin Shin, Seok Chung, Hyo Jeong Kuh

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Multicellular 3D culture and interaction with stromal components are considered essential elements in establishing a 'more clinically relevant' tumor model. Matrix-embedded 3D cultures using a microfluidic chip platform can recapitulate the microscale interaction within tumor microenvironments. As a major component of tumor microenvironment, cancer-associated fibroblasts (CAFs) play a role in cancer progression and drug resistance. Here, we present a microfluidic chip-based tumor tissue culture model that integrates 3D tumor spheroids (TSs) with CAF in proximity within a hydrogel scaffold. HT-29 human colorectal carcinoma cells grew into 3D TSs and the growth was stimulated when co-cultured with fibroblasts as shown by 1.5-folds increase of % changes in diameter over 5 days. TS cultured for 6 days showed a reduced expression of Ki-67 along with increased expression of fibronectin when co-cultured with fibroblasts compared to mono-cultured TSs. Fibroblasts were activated under co-culture conditions, as demonstrated by increases in α-SMA expression and migratory activity. When exposed to paclitaxel, a survival advantage was observed in TSs co-cultured with activated fibroblasts. Overall, we demonstrated the reciprocal interaction between TSs and fibroblasts in our 7-channel microfluidic chip. The co-culture of 3D TS-CAF in a collagen matrix-incorporated microfluidic chip may be useful to study the tumor microenvironment and for evaluation of drug screening and evaluation.

Original languageEnglish
Article numbere0159013
JournalPLoS One
Volume11
Issue number7
DOIs
Publication statusPublished - 2016 Jul 1

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Tumor Microenvironment
Microfluidics
coculture
Fibroblasts
Coculture Techniques
Cell culture
fibroblasts
collagen
Tumors
Collagen
Chemical activation
neoplasms
Neoplasms
Preclinical Drug Evaluations
Drug Evaluation
Hydrogel
Tissue culture
Paclitaxel
Fibronectins
Drug Resistance

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Co-culture of tumor spheroids and fibroblasts in a collagen matrix-incorporated microfluidic chip mimics reciprocal activation in solid tumor microenvironment. / Jeong, Su Yeong; Lee, Ji Hyun; Shin, Yoojin; Chung, Seok; Kuh, Hyo Jeong.

In: PLoS One, Vol. 11, No. 7, e0159013, 01.07.2016.

Research output: Contribution to journalArticle

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