Co-expression of MDR1 and HLA-B7 Genes in a Mammalian Cell Using a Retrovirus

Seong Min Lee, Kyoo Hyung Lee, Hag Dong Kim, Je Hwan Lee, Jung Shin Lee, Joon Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatlbility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDR1 gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, this virus will be used to infect hematopoietic stem cells to co-express MDR1 and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.

Original languageEnglish
Pages (from-to)176-181
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume34
Issue number2
Publication statusPublished - 2001 Mar 31

Fingerprint

HLA-B7 Antigen
Retroviridae
Genes
Cells
Product Packaging
Viruses
Packaging
Encephalomyocarditis virus
MHC Class I Genes
MDR Genes
Lymphocytes
Viral RNA
Vincristine
Transcription
Fibroblasts
Chromosomes
Hematopoietic Stem Cells
Stem cells
Reverse Transcription

Keywords

  • Hematopoietic stem cell
  • HLA-B7
  • MDR1
  • PA317
  • Retrovirus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Co-expression of MDR1 and HLA-B7 Genes in a Mammalian Cell Using a Retrovirus. / Lee, Seong Min; Lee, Kyoo Hyung; Kim, Hag Dong; Lee, Je Hwan; Lee, Jung Shin; Kim, Joon.

In: Journal of Biochemistry and Molecular Biology, Vol. 34, No. 2, 31.03.2001, p. 176-181.

Research output: Contribution to journalArticle

Lee, Seong Min ; Lee, Kyoo Hyung ; Kim, Hag Dong ; Lee, Je Hwan ; Lee, Jung Shin ; Kim, Joon. / Co-expression of MDR1 and HLA-B7 Genes in a Mammalian Cell Using a Retrovirus. In: Journal of Biochemistry and Molecular Biology. 2001 ; Vol. 34, No. 2. pp. 176-181.
@article{8bc39e847d6e454f8ee04e458fcb15a0,
title = "Co-expression of MDR1 and HLA-B7 Genes in a Mammalian Cell Using a Retrovirus",
abstract = "Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatlbility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDR1 gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, this virus will be used to infect hematopoietic stem cells to co-express MDR1 and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.",
keywords = "Hematopoietic stem cell, HLA-B7, MDR1, PA317, Retrovirus",
author = "Lee, {Seong Min} and Lee, {Kyoo Hyung} and Kim, {Hag Dong} and Lee, {Je Hwan} and Lee, {Jung Shin} and Joon Kim",
year = "2001",
month = "3",
day = "31",
language = "English",
volume = "34",
pages = "176--181",
journal = "BMB Reports",
issn = "1976-6696",
publisher = "The Biochemical Society of the Republic of Korea",
number = "2",

}

TY - JOUR

T1 - Co-expression of MDR1 and HLA-B7 Genes in a Mammalian Cell Using a Retrovirus

AU - Lee, Seong Min

AU - Lee, Kyoo Hyung

AU - Kim, Hag Dong

AU - Lee, Je Hwan

AU - Lee, Jung Shin

AU - Kim, Joon

PY - 2001/3/31

Y1 - 2001/3/31

N2 - Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatlbility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDR1 gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, this virus will be used to infect hematopoietic stem cells to co-express MDR1 and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.

AB - Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatlbility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDR1 gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, this virus will be used to infect hematopoietic stem cells to co-express MDR1 and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.

KW - Hematopoietic stem cell

KW - HLA-B7

KW - MDR1

KW - PA317

KW - Retrovirus

UR - http://www.scopus.com/inward/record.url?scp=0035590637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035590637&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0035590637

VL - 34

SP - 176

EP - 181

JO - BMB Reports

JF - BMB Reports

SN - 1976-6696

IS - 2

ER -