Co-fermentation of carbon sources by Enterobacter aerogenes ATCC 29007 to enhance the production of bioethanol

Laxmi Prasad Thapa, Sang Jun Lee, Xiao Guang Yang, Hah Young Yoo, Sung Bong Kim, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We investigated the enhancement of bioethanol production in Enterobacter aerogenes ATCC 29007 by cofermentation of carbon sources such as glycerol, glucose, galactose, sucrose, fructose, xylose, starch, mannitol and citric acid. Biofuel production increases with increasing growth rate of microorganisms; that is why we investigated the optimal growth rate of E. aerogenes ATCC 29007, using mixtures of different carbon sources with glycerol. E. aerogenes ATCC 29007 was incubated in media containing each carbon source and glycerol; growth rate and bioethanol production improved in all cases compared to those in medium containing glycerol alone. The growth rate and bioethanol production were highest with mannitol. Fermentation was carried out at 37 °C for 18 h, pH 7, using 50 mL defined production medium in 100 mL serum bottles at 200 rpm. Bioethanol production under optimized conditions in medium containing 16 g/L mannitol and 20 g/L glycerol increased sixfold (32.10 g/L) than that containing glycerol alone (5.23 g/L) as the carbon source in anaerobic conditions. Similarly, bioethanol production using free cells in continuous co-fermentation also improved (27.28 g/L) when 90.37 % of 16 g/L mannitol and 67.15 % of 20 g/L glycerol were used. Although naturally existing or engineered microorganisms can ferment mixed sugars sequentially, the preferential utilization of glucose to non-glucose sugars often results in lower overall yield and productivity of ethanol. Here, we present new findings in E. aerogenes ATCC 29007 that can be used to improve bioethanol production by simultaneous co-fermentation of glycerol and mannitol.

Original languageEnglish
Pages (from-to)1073-1084
Number of pages12
JournalBioprocess and Biosystems Engineering
Volume37
Issue number6
DOIs
Publication statusPublished - 2014 Jan 1

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Enterobacter aerogenes
Bioethanol
Glycerol
Fermentation
Carbon
Mannitol
Growth
Sugars
Microorganisms
Glucose
Fructose
Xylose
Biofuels
Citric acid
Bottles
Sugar (sucrose)
Starch
Galactose
Citric Acid
Sucrose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Co-fermentation of carbon sources by Enterobacter aerogenes ATCC 29007 to enhance the production of bioethanol. / Thapa, Laxmi Prasad; Lee, Sang Jun; Yang, Xiao Guang; Yoo, Hah Young; Kim, Sung Bong; Park, Chulhwan; Kim, Seung Wook.

In: Bioprocess and Biosystems Engineering, Vol. 37, No. 6, 01.01.2014, p. 1073-1084.

Research output: Contribution to journalArticle

Thapa, Laxmi Prasad ; Lee, Sang Jun ; Yang, Xiao Guang ; Yoo, Hah Young ; Kim, Sung Bong ; Park, Chulhwan ; Kim, Seung Wook. / Co-fermentation of carbon sources by Enterobacter aerogenes ATCC 29007 to enhance the production of bioethanol. In: Bioprocess and Biosystems Engineering. 2014 ; Vol. 37, No. 6. pp. 1073-1084.
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