Abstract
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
Original language | English |
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Pages (from-to) | 1335-1338 |
Number of pages | 4 |
Journal | Korean Journal of Chemical Engineering |
Volume | 30 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2013 Jun |
Keywords
- Biodiesel
- Co-immobilization
- Enzymatic Process
- Lipase
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)