Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

Jong Ho Lee, Sung Bong Kim, Hah Young Yoo, Ja Hyun Lee, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.

Original languageEnglish
Pages (from-to)1335-1338
Number of pages4
JournalKorean Journal of Chemical Engineering
Issue number6
Publication statusPublished - 2013 Jun


  • Biodiesel
  • Co-immobilization
  • Enzymatic Process
  • Lipase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)


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