Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

Jong Ho Lee, Sung Bong Kim, Hah Young Yoo, Ja Hyun Lee, Sung Ok Han, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.

Original languageEnglish
Pages (from-to)1335-1338
Number of pages4
JournalKorean Journal of Chemical Engineering
Volume30
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Fingerprint

Immobilized Enzymes
Biofuels
Candida
Lipases
Biodiesel
Lipase
Methanol
Enzymes
Silica Gel
Packed beds
Silica gel

Keywords

  • Biodiesel
  • Co-immobilization
  • Enzymatic Process
  • Lipase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production. / Lee, Jong Ho; Kim, Sung Bong; Yoo, Hah Young; Lee, Ja Hyun; Han, Sung Ok; Park, Chulhwan; Kim, Seung Wook.

In: Korean Journal of Chemical Engineering, Vol. 30, No. 6, 01.06.2013, p. 1335-1338.

Research output: Contribution to journalArticle

Lee, Jong Ho ; Kim, Sung Bong ; Yoo, Hah Young ; Lee, Ja Hyun ; Han, Sung Ok ; Park, Chulhwan ; Kim, Seung Wook. / Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production. In: Korean Journal of Chemical Engineering. 2013 ; Vol. 30, No. 6. pp. 1335-1338.
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