Abstract
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
| Original language | English |
|---|---|
| Pages (from-to) | 1335-1338 |
| Number of pages | 4 |
| Journal | Korean Journal of Chemical Engineering |
| Volume | 30 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2013 Jun 1 |
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Keywords
- Biodiesel
- Co-immobilization
- Enzymatic Process
- Lipase
ASJC Scopus subject areas
- Chemistry(all)
- Chemical Engineering(all)
Cite this
Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production. / Lee, Jong Ho; Kim, Sung Bong; Yoo, Hah Young; Lee, Ja Hyun; Han, Sung Ok; Park, Chulhwan; Kim, Seung Wook.
In: Korean Journal of Chemical Engineering, Vol. 30, No. 6, 01.06.2013, p. 1335-1338.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production
AU - Lee, Jong Ho
AU - Kim, Sung Bong
AU - Yoo, Hah Young
AU - Lee, Ja Hyun
AU - Han, Sung Ok
AU - Park, Chulhwan
AU - Kim, Seung Wook
PY - 2013/6/1
Y1 - 2013/6/1
N2 - Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
AB - Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
KW - Biodiesel
KW - Co-immobilization
KW - Enzymatic Process
KW - Lipase
UR - http://www.scopus.com/inward/record.url?scp=84878531615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878531615&partnerID=8YFLogxK
U2 - 10.1007/s11814-013-0058-z
DO - 10.1007/s11814-013-0058-z
M3 - Article
VL - 30
SP - 1335
EP - 1338
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
SN - 0256-1115
IS - 6
ER -