Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes

Insu Kim, Dongtak Lee, Sang Won Lee, Jeong Hoon Lee, Gyudo Lee, Dae Sung Yoon

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.

Original languageEnglish
Pages (from-to)6386-6394
Number of pages9
JournalACS nano
Volume15
Issue number4
DOIs
Publication statusPublished - 2021 Apr 27

Keywords

  • cardiovascular disease
  • coagulation-inspired
  • integrin αβ
  • localized surface plasmon resonance
  • red blood cell membrane

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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