Codonopsis lanceolata Contributes to Ca 2+Homeostasis by Mediating SOCE and PLC/IP 3Pathways in Vascular Endothelial and Smooth Muscle Cells

Min Kyung Kim, A. Young Han, You Kyoung Shin, Kwang Won Lee, Geun Hee Seol

Research output: Contribution to journalArticlepeer-review

Abstract

Codonopsis lanceolata has been widely used as an anti-inflammatory and anti-lipogenic agent in traditional medicine. Recently, C. lanceolata was reported to prevent hypertension by improving vascular function. This study evaluated the effects of C. lanceolata and its major component lancemaside A on cytosolic calcium concentration in vascular endothelial cells and vascular smooth muscle cells. Cytosolic calcium concentration was measured using fura-2 AM fluorescence. C. lanceolata or lancemaside A increased the cytosolic calcium concentration by releasing Ca 2+from the endoplasmic reticulum and sarcoplasmic reticulum and by Ca 2+entry into endothelial cells and vascular smooth muscle cells from extracellular sources. The C. lanceolata - and lancemaside A-induced cytosolic calcium concentration increases were significantly inhibited by lanthanum, an inhibitor of non-selective cation channels, in both endothelial cells and vascular smooth muscle cells. Moreover, C. lanceolata and lancemaside A significantly inhibited store-operated Ca 2+entry under pathological extracellular Ca 2+levels. In Ca 2+-free extracellular fluid, increases in the cytosolic calcium concentration induced by C. lanceolata or lancemaside A were significantly inhibited by U73122, an inhibitor of phospholipase C, and 2-APB, an inositol 1,4,5-trisphosphate receptor antagonist. In addition, dantrolene treatment, which inhibits Ca 2+release through ryanodine receptor channels, also inhibited C. lanceolata - or lancemaside A-induced increases in the cytosolic calcium concentration through the phospholipase C/inositol 1,4,5-trisphosphate pathway. These results suggest that C. lanceolata and lancemaside A increase the cytosolic calcium concentration through the non-selective cation channels and phospholipase C/inositol 1,4,5-trisphosphate pathways under physiological conditions and inhibit store-operated Ca 2+entry under pathological conditions in endothelial cells and vascular smooth muscle cells. C. lanceolata or lancemaside A can protect endothelial cells and vascular smooth muscle cells by maintaining cytosolic calcium concentration homeostasis, suggesting possible applications for these materials in diets for preventing vascular damage.

Original languageEnglish
JournalPlanta Medica
DOIs
Publication statusAccepted/In press - 2020

Keywords

  • Campanulaceae
  • Codonopsis lanceolate
  • inositol 1,4,5-trisphosphate
  • store-operated Ca entry
  • vascular endothelial cells
  • vascular smooth muscle cells

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Codonopsis lanceolata Contributes to Ca <sup>2+</sup>Homeostasis by Mediating SOCE and PLC/IP <sub>3</sub>Pathways in Vascular Endothelial and Smooth Muscle Cells'. Together they form a unique fingerprint.

Cite this