Cognate CD4 help is essential for the reactivation and expansion of CD8 memory T cells directed against the hematopoietic cell-specific dominant minor histocompatibility antigen, H60

Jeong Ryu Su, Min Jung Kyung, Seung Yoo Hyun, Tae Woo Kim, Kim Sol, Chang Jun, Young Choi Eun

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In contrast to previous notions of the help-independency of memory CD8 T cells during secondary expansion, here we show that CD4 help is indispensable for the re-expansion of once-helped memory CD8 T cells, using a hematopoietic cell-specific dominant minor histocompatibility (H) antigen, H60, as a model antigen. H60-specific memory CD8 T cells generated during a helped primary response vigorously expanded only when rechallenged under helped conditions. The help requirement for an optimal secondary response was confirmed by a reduction in peak size by CD4 depletion, and was reproduced after skin transplantation. Helpless conditions or noncognate separate help during the secondary response resulted in a significant reduction in the peak size and different response kinetics. Providing CD4 help again during a tertiary challenge restored robust memory expansion; however, the repeated deprivation of help further reduced clonal expansion. Adoptively transferred memory CD8 T cells did not proliferate in CD40L-/- hosts. In the CD40-/- hosts, marginal memory expansion was detected after priming with male H60 cells but was completely abolished by priming with peptide-loaded CD40-/- cells, suggesting the essential role of CD40 and CD40L in memory responses. These results provide insight into the control of minor H antigen-specific CD8 T-cell responses, to maximize the graft-versus-leukemia response.

Original languageEnglish
Pages (from-to)4273-4280
Number of pages8
JournalBlood
Volume113
Issue number18
DOIs
Publication statusPublished - 2009 Nov 18

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Minor Histocompatibility Antigens
T-cells
T-Lymphocytes
Data storage equipment
CD40 Ligand
Skin Transplantation
Leukemia
Grafts
Transplants
Antigens
Peptides
Skin
Kinetics

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Cognate CD4 help is essential for the reactivation and expansion of CD8 memory T cells directed against the hematopoietic cell-specific dominant minor histocompatibility antigen, H60. / Su, Jeong Ryu; Kyung, Min Jung; Hyun, Seung Yoo; Kim, Tae Woo; Sol, Kim; Jun, Chang; Eun, Young Choi.

In: Blood, Vol. 113, No. 18, 18.11.2009, p. 4273-4280.

Research output: Contribution to journalArticle

Su, Jeong Ryu ; Kyung, Min Jung ; Hyun, Seung Yoo ; Kim, Tae Woo ; Sol, Kim ; Jun, Chang ; Eun, Young Choi. / Cognate CD4 help is essential for the reactivation and expansion of CD8 memory T cells directed against the hematopoietic cell-specific dominant minor histocompatibility antigen, H60. In: Blood. 2009 ; Vol. 113, No. 18. pp. 4273-4280.
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