Cohnella candidum sp. nov., radiation-resistant bacterium from soil

Soo Hyun Maeng, Myung Kyum Kim, Jun Hwee Jang, Hana Yi, Gayathri Subramani

Research output: Contribution to journalArticle

Abstract

A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7 T , was isolated from soil collected in Jeju Island, South Korea. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%). Optimal growth occurred at 30 °C, pH 6.5 and in the absence of NaCl. The predominant cellular fatty acids were anteiso-C 15:0 and iso-C 16:0 . The major respiratory quinone was MK-7. The polar lipids profile comprised of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The DNA G + C content was 57.0 mol %. The genotypic and phenotypic analyses revealed the differentiation of strain 18JY8-7 T from all recognized Cohenella species. The strain 18JY8-7 T , therefore represents a novel bacterial species within the family Paenibacillaceae, for which the name Cohnella candidum sp. nov. is proposed. The type strain is 18JY8-7 T (= KCTC 33969 T = JCM 33199 T ).

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Bacillales
Republic of Korea
Phosphatidylglycerols
Cardiolipins
Base Composition
rRNA Genes
Islands
Bacillus
Names
Fatty Acids
Soil
Radiation
Bacteria
Lipids
DNA
Growth
Gram's stain
phosphatidylethanolamine
benzoquinone
C 15

Keywords

  • Cohnella
  • Novel species
  • Paenibacillaceae
  • Radiation-resistant
  • Taxonomy

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Cohnella candidum sp. nov., radiation-resistant bacterium from soil. / Maeng, Soo Hyun; Kim, Myung Kyum; Jang, Jun Hwee; Yi, Hana; Subramani, Gayathri.

In: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7 T , was isolated from soil collected in Jeju Island, South Korea. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1{\%}, sequence similarity) and Cohnella xylanilytica (96.0{\%}). Optimal growth occurred at 30 °C, pH 6.5 and in the absence of NaCl. The predominant cellular fatty acids were anteiso-C 15:0 and iso-C 16:0 . The major respiratory quinone was MK-7. The polar lipids profile comprised of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The DNA G + C content was 57.0 mol {\%}. The genotypic and phenotypic analyses revealed the differentiation of strain 18JY8-7 T from all recognized Cohenella species. The strain 18JY8-7 T , therefore represents a novel bacterial species within the family Paenibacillaceae, for which the name Cohnella candidum sp. nov. is proposed. The type strain is 18JY8-7 T (= KCTC 33969 T = JCM 33199 T ).",
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AB - A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7 T , was isolated from soil collected in Jeju Island, South Korea. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%). Optimal growth occurred at 30 °C, pH 6.5 and in the absence of NaCl. The predominant cellular fatty acids were anteiso-C 15:0 and iso-C 16:0 . The major respiratory quinone was MK-7. The polar lipids profile comprised of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The DNA G + C content was 57.0 mol %. The genotypic and phenotypic analyses revealed the differentiation of strain 18JY8-7 T from all recognized Cohenella species. The strain 18JY8-7 T , therefore represents a novel bacterial species within the family Paenibacillaceae, for which the name Cohnella candidum sp. nov. is proposed. The type strain is 18JY8-7 T (= KCTC 33969 T = JCM 33199 T ).

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