Cold atmospheric plasma (CAP), a novel physicochemical source, induces neural differentiation through cross-talk between the specific RONS cascade and Trk/Ras/ERK signaling pathway

Ja Young Jang, Young June Hong, Junsup Lim, Jin Sung Choi, Eun Ha Choi, Seong Man Kang, Hyangshuk Rhim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Plasma, formed by ionization of gas molecules or atoms, is the most abundant form of matter and consists of highly reactive physicochemical species. In the physics and chemistry fields, plasma has been extensively studied; however, the exact action mechanisms of plasma on biological systems, including cells and humans, are not well known. Recent evidence suggests that cold atmospheric plasma (CAP), which refers to plasma used in the biomedical field, may regulate diverse cellular processes, including neural differentiation. However, the mechanism by which these physicochemical signals, elicited by reactive oxygen and nitrogen species (RONS), are transmitted to biological system remains elusive. In this study, we elucidated the physicochemical and biological (PCB) connection between the CAP cascade and Trk/Ras/ERK signaling pathway, which resulted in neural differentiation. Excited atomic oxygen in the plasma phase led to the formation of RONS in the PCB network, which then interacted with reactive atoms in the extracellular liquid phase to form nitric oxide (NO). Production of large amounts of superoxide radical ([rad]O2 ) in the mitochondria of cells exposed to CAP demonstrated that extracellular NO induced the reversible inhibition of mitochondrial complex IV. We also demonstrated that cytosolic hydrogen peroxide, formed by [rad]O2 dismutation, act as an intracellular messenger to specifically activate the Trk/Ras/ERK signaling pathway. This study is the first to elucidate the mechanism linking physicochemical signals from the CAP cascade to the intracellular neural differentiation signaling pathway, providing physical, chemical and biological insights into the development of therapeutic techniques to treat neurological diseases.

Original languageEnglish
Pages (from-to)258-273
Number of pages16
JournalBiomaterials
Volume156
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Plasma Gases
Reactive Nitrogen Species
MAP Kinase Signaling System
Reactive Oxygen Species
Nitrogen
Plasmas
Oxygen
Nitric Oxide
Nitric oxide
Biological systems
Physics
Superoxides
Hydrogen Peroxide
Mitochondria
Ionization of gases
Gases
Atoms
Hydrogen peroxide

Keywords

  • Cold atmospheric plasma (CAP)
  • Neural differentiation
  • Neurological disease
  • Plasma
  • Reactive oxygen and nitrogen species (RONS)

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Cold atmospheric plasma (CAP), a novel physicochemical source, induces neural differentiation through cross-talk between the specific RONS cascade and Trk/Ras/ERK signaling pathway. / Jang, Ja Young; Hong, Young June; Lim, Junsup; Choi, Jin Sung; Choi, Eun Ha; Kang, Seong Man; Rhim, Hyangshuk.

In: Biomaterials, Vol. 156, 01.02.2018, p. 258-273.

Research output: Contribution to journalArticle

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