Collagen architecture in pregnancy-induced protection from breast cancer

Ori Maller, Kirk C. Hansen, Traci R. Lyons, Irene Acerbi, Valerie M. Weaver, Rytis Prekeris, Aik-Choon Tan, Pepper Schedin

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The reduction in breast cancer risk attributed to early-age pregnancy is mediated in part by changes in the mammary epithelium. Here, we address the role of the mammary stroma in this protection. Utilizing tumor cells capable of transitioning from indolent to proliferative or invasive states, we demonstrate that mammary extracellular matrix (ECM) from parous rats (parous matrix) decreases tumor growth and impedes cellular phenotypes associated with tumor cell invasion compared with that observed using nulliparous matrix. Proteomic analysis identifies an increased abundance of collagen I in parous matrix, an observation extended to breast tissue of parous women. Given the pro-tumorigenic attributes of fibrillar collagen, these results were unexpected. Second-harmonic generation imaging and atomic force microscopy revealed that the abundant collagen observed in the mammary glands of parous rats is less linearized and associated with a decrease in stromal stiffness, implicating collagen organization and stiffness in parity-induced protection. Using 3D cell culture models, we demonstrate that linearized (fibrillar) collagen I induces cellular phenotypes consistent with an invasive behavior in mammary tumor cells and alters the subcellular distribution of b1 integrin. Conversely, high-density non-fibrillar collagen I induces tumor-suppressive attributes, including increases in junctional E-cadherin in tumor cells, upregulation of genes encoding components of cell-cell junctions, and downregulation of mesenchymal-specific and metalloproteinase-encoding genes. These data show that collagen organization, rather than density alone, is a key contributor to the invasive phenotype. Furthermore, our data show that parity alters the composition and organization of mammary ECM, particularly fibrillar collagen, in a manner consistent with tumor suppression.

Original languageEnglish
Pages (from-to)4108-4120
Number of pages13
JournalJournal of Cell Science
Volume126
Issue number18
DOIs
Publication statusPublished - 2013 Oct 18
Externally publishedYes

Fingerprint

Collagen
Fibrillar Collagens
Breast Neoplasms
Breast
Pregnancy
Neoplasms
Parity
Phenotype
Extracellular Matrix
Non-Fibrillar Collagens
Gene Components
Intercellular Junctions
Atomic Force Microscopy
Metalloproteases
Cadherins
Human Mammary Glands
Integrins
Proteomics
Up-Regulation
Down-Regulation

Keywords

  • Collagen organization
  • E-cadherin
  • ECM
  • Integrin signaling
  • Tumor-suppressive extracellular matrix

ASJC Scopus subject areas

  • Cell Biology

Cite this

Maller, O., Hansen, K. C., Lyons, T. R., Acerbi, I., Weaver, V. M., Prekeris, R., ... Schedin, P. (2013). Collagen architecture in pregnancy-induced protection from breast cancer. Journal of Cell Science, 126(18), 4108-4120. https://doi.org/10.1242/jcs.121590

Collagen architecture in pregnancy-induced protection from breast cancer. / Maller, Ori; Hansen, Kirk C.; Lyons, Traci R.; Acerbi, Irene; Weaver, Valerie M.; Prekeris, Rytis; Tan, Aik-Choon; Schedin, Pepper.

In: Journal of Cell Science, Vol. 126, No. 18, 18.10.2013, p. 4108-4120.

Research output: Contribution to journalArticle

Maller, O, Hansen, KC, Lyons, TR, Acerbi, I, Weaver, VM, Prekeris, R, Tan, A-C & Schedin, P 2013, 'Collagen architecture in pregnancy-induced protection from breast cancer', Journal of Cell Science, vol. 126, no. 18, pp. 4108-4120. https://doi.org/10.1242/jcs.121590
Maller O, Hansen KC, Lyons TR, Acerbi I, Weaver VM, Prekeris R et al. Collagen architecture in pregnancy-induced protection from breast cancer. Journal of Cell Science. 2013 Oct 18;126(18):4108-4120. https://doi.org/10.1242/jcs.121590
Maller, Ori ; Hansen, Kirk C. ; Lyons, Traci R. ; Acerbi, Irene ; Weaver, Valerie M. ; Prekeris, Rytis ; Tan, Aik-Choon ; Schedin, Pepper. / Collagen architecture in pregnancy-induced protection from breast cancer. In: Journal of Cell Science. 2013 ; Vol. 126, No. 18. pp. 4108-4120.
@article{1750a2445ed441e28e22937addb77a1d,
title = "Collagen architecture in pregnancy-induced protection from breast cancer",
abstract = "The reduction in breast cancer risk attributed to early-age pregnancy is mediated in part by changes in the mammary epithelium. Here, we address the role of the mammary stroma in this protection. Utilizing tumor cells capable of transitioning from indolent to proliferative or invasive states, we demonstrate that mammary extracellular matrix (ECM) from parous rats (parous matrix) decreases tumor growth and impedes cellular phenotypes associated with tumor cell invasion compared with that observed using nulliparous matrix. Proteomic analysis identifies an increased abundance of collagen I in parous matrix, an observation extended to breast tissue of parous women. Given the pro-tumorigenic attributes of fibrillar collagen, these results were unexpected. Second-harmonic generation imaging and atomic force microscopy revealed that the abundant collagen observed in the mammary glands of parous rats is less linearized and associated with a decrease in stromal stiffness, implicating collagen organization and stiffness in parity-induced protection. Using 3D cell culture models, we demonstrate that linearized (fibrillar) collagen I induces cellular phenotypes consistent with an invasive behavior in mammary tumor cells and alters the subcellular distribution of b1 integrin. Conversely, high-density non-fibrillar collagen I induces tumor-suppressive attributes, including increases in junctional E-cadherin in tumor cells, upregulation of genes encoding components of cell-cell junctions, and downregulation of mesenchymal-specific and metalloproteinase-encoding genes. These data show that collagen organization, rather than density alone, is a key contributor to the invasive phenotype. Furthermore, our data show that parity alters the composition and organization of mammary ECM, particularly fibrillar collagen, in a manner consistent with tumor suppression.",
keywords = "Collagen organization, E-cadherin, ECM, Integrin signaling, Tumor-suppressive extracellular matrix",
author = "Ori Maller and Hansen, {Kirk C.} and Lyons, {Traci R.} and Irene Acerbi and Weaver, {Valerie M.} and Rytis Prekeris and Aik-Choon Tan and Pepper Schedin",
year = "2013",
month = "10",
day = "18",
doi = "10.1242/jcs.121590",
language = "English",
volume = "126",
pages = "4108--4120",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "18",

}

TY - JOUR

T1 - Collagen architecture in pregnancy-induced protection from breast cancer

AU - Maller, Ori

AU - Hansen, Kirk C.

AU - Lyons, Traci R.

AU - Acerbi, Irene

AU - Weaver, Valerie M.

AU - Prekeris, Rytis

AU - Tan, Aik-Choon

AU - Schedin, Pepper

PY - 2013/10/18

Y1 - 2013/10/18

N2 - The reduction in breast cancer risk attributed to early-age pregnancy is mediated in part by changes in the mammary epithelium. Here, we address the role of the mammary stroma in this protection. Utilizing tumor cells capable of transitioning from indolent to proliferative or invasive states, we demonstrate that mammary extracellular matrix (ECM) from parous rats (parous matrix) decreases tumor growth and impedes cellular phenotypes associated with tumor cell invasion compared with that observed using nulliparous matrix. Proteomic analysis identifies an increased abundance of collagen I in parous matrix, an observation extended to breast tissue of parous women. Given the pro-tumorigenic attributes of fibrillar collagen, these results were unexpected. Second-harmonic generation imaging and atomic force microscopy revealed that the abundant collagen observed in the mammary glands of parous rats is less linearized and associated with a decrease in stromal stiffness, implicating collagen organization and stiffness in parity-induced protection. Using 3D cell culture models, we demonstrate that linearized (fibrillar) collagen I induces cellular phenotypes consistent with an invasive behavior in mammary tumor cells and alters the subcellular distribution of b1 integrin. Conversely, high-density non-fibrillar collagen I induces tumor-suppressive attributes, including increases in junctional E-cadherin in tumor cells, upregulation of genes encoding components of cell-cell junctions, and downregulation of mesenchymal-specific and metalloproteinase-encoding genes. These data show that collagen organization, rather than density alone, is a key contributor to the invasive phenotype. Furthermore, our data show that parity alters the composition and organization of mammary ECM, particularly fibrillar collagen, in a manner consistent with tumor suppression.

AB - The reduction in breast cancer risk attributed to early-age pregnancy is mediated in part by changes in the mammary epithelium. Here, we address the role of the mammary stroma in this protection. Utilizing tumor cells capable of transitioning from indolent to proliferative or invasive states, we demonstrate that mammary extracellular matrix (ECM) from parous rats (parous matrix) decreases tumor growth and impedes cellular phenotypes associated with tumor cell invasion compared with that observed using nulliparous matrix. Proteomic analysis identifies an increased abundance of collagen I in parous matrix, an observation extended to breast tissue of parous women. Given the pro-tumorigenic attributes of fibrillar collagen, these results were unexpected. Second-harmonic generation imaging and atomic force microscopy revealed that the abundant collagen observed in the mammary glands of parous rats is less linearized and associated with a decrease in stromal stiffness, implicating collagen organization and stiffness in parity-induced protection. Using 3D cell culture models, we demonstrate that linearized (fibrillar) collagen I induces cellular phenotypes consistent with an invasive behavior in mammary tumor cells and alters the subcellular distribution of b1 integrin. Conversely, high-density non-fibrillar collagen I induces tumor-suppressive attributes, including increases in junctional E-cadherin in tumor cells, upregulation of genes encoding components of cell-cell junctions, and downregulation of mesenchymal-specific and metalloproteinase-encoding genes. These data show that collagen organization, rather than density alone, is a key contributor to the invasive phenotype. Furthermore, our data show that parity alters the composition and organization of mammary ECM, particularly fibrillar collagen, in a manner consistent with tumor suppression.

KW - Collagen organization

KW - E-cadherin

KW - ECM

KW - Integrin signaling

KW - Tumor-suppressive extracellular matrix

UR - http://www.scopus.com/inward/record.url?scp=84885451271&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885451271&partnerID=8YFLogxK

U2 - 10.1242/jcs.121590

DO - 10.1242/jcs.121590

M3 - Article

VL - 126

SP - 4108

EP - 4120

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 18

ER -