Collagen-based brain microvasculature model in vitro using three-dimensional printed template

Jeong Ah Kim, Hong Nam Kim, Sun Kyoung Im, Seok Chung, Ji Yoon Kang, Nakwon Choi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We present an engineered three-dimensional (3D) in vitro brain microvasculature system embedded within the bulk of a collagen matrix. To create a hydrogel template for the functional brain microvascular structure, we fabricated an array of microchannels made of collagen I using microneedles and a 3D printed frame. By culturing mouse brain endothelial cells (bEnd.3) on the luminal surface of cylindrical collagen microchannels, we reconstructed an array of brain microvasculature in vitro with circular cross-sections. We characterized the barrier function of our brain microvasculature by measuring transendothelial permeability of 40 kDa fluorescein isothiocyanate-dextran (Stoke's radius of ~4.5 nm), based on an analytical model. The transendothelial permeability decreased significantly over 3 weeks of culture. We also present the disruption of the barrier function with a hyperosmotic mannitol as well as a subsequent recovery over 4 days. Our brain microvasculature model in vitro, consisting of system-in-hydrogel combined with the widely emerging 3D printing technique, can serve as a useful tool not only for fundamental studies associated with blood-brain barrier in physiological and pathological settings but also for pharmaceutical applications.

Original languageEnglish
Article number1.4917508
JournalBiomicrofluidics
Volume9
Issue number2
DOIs
Publication statusPublished - 2015 Apr 15

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Brain models
collagens
Microvessels
Collagen
brain
Brain
templates
Hydrogel
Microchannels
Hydrogels
microchannels
Permeability
permeability
Dextran
mannitol
blood-brain barrier
Endothelial cells
Mannitol
dextrans
Embedded systems

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

Collagen-based brain microvasculature model in vitro using three-dimensional printed template. / Kim, Jeong Ah; Kim, Hong Nam; Im, Sun Kyoung; Chung, Seok; Kang, Ji Yoon; Choi, Nakwon.

In: Biomicrofluidics, Vol. 9, No. 2, 1.4917508, 15.04.2015.

Research output: Contribution to journalArticle

Kim, Jeong Ah ; Kim, Hong Nam ; Im, Sun Kyoung ; Chung, Seok ; Kang, Ji Yoon ; Choi, Nakwon. / Collagen-based brain microvasculature model in vitro using three-dimensional printed template. In: Biomicrofluidics. 2015 ; Vol. 9, No. 2.
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