Collagen scaffolds derived from a marine source and their biocompatibility

Eun Song, So Yeon Kim, Taehoon Chun, Hyun Jung Byun, Young Moo Lee

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

The primary sources of industrial collagens are calf skin and bone. However, these carry a high risk of bovine spongiform encephalopathy or transmissible spongiform encephalopathy. In this study, a novel form of acid-soluble collagen was extracted from jellyfish in an effort to obtain an alternative and safer collagen. Porous scaffolds composed of jellyfish collagen were prepared by freeze-drying and cross-linking with 1-ethyl-(3-3- dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide to be used in tissue engineering applications. Enzymatic degradation kinetics of jellyfish collagen scaffolds were controlled by EDC/NHS-cross-linking density. Results from an MTT assay indicated that jellyfish collagen exhibited higher cell viability than other naturally derived biomaterials, including bovine collagen, gelatin, hyaluronic acid, and glucan. Jellyfish collagen scaffolds also had a highly porous and interconnected pore structure, which is useful for an high-density cell seeding, an efficient nutrient and an oxygen supply to the cells cultured in the three-dimensional matrices. To determine whether jellyfish collagen evokes any specific inflammatory response compared to that induced by bovine collagen or gelatin, we measured the levels of pro-inflammatory cytokines and antibody secretions and monitored the population changes of immune cells after in vivo implantation. Jellyfish collagen was found to induce an immune response at least comparable to those caused by bovine collagen and gelatin.

Original languageEnglish
Pages (from-to)2951-2961
Number of pages11
JournalBiomaterials
Volume27
Issue number15
DOIs
Publication statusPublished - 2006 May 1
Externally publishedYes

Fingerprint

Scaffolds (biology)
Biocompatibility
Collagen
Gelatin
Scaffolds
Ethyldimethylaminopropyl Carbodiimide
Bovine Spongiform Encephalopathy
Oxygen supply
Hyaluronic acid
Prion Diseases
Freeze Drying
Glucans
Biocompatible Materials
Hyaluronic Acid
Tissue Engineering
Pore structure
Tissue engineering
Antibodies
Biomaterials
Nutrients

Keywords

  • Collagen
  • Immune response
  • Jellyfish
  • Scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Collagen scaffolds derived from a marine source and their biocompatibility. / Song, Eun; Yeon Kim, So; Chun, Taehoon; Byun, Hyun Jung; Lee, Young Moo.

In: Biomaterials, Vol. 27, No. 15, 01.05.2006, p. 2951-2961.

Research output: Contribution to journalArticle

Song, Eun ; Yeon Kim, So ; Chun, Taehoon ; Byun, Hyun Jung ; Lee, Young Moo. / Collagen scaffolds derived from a marine source and their biocompatibility. In: Biomaterials. 2006 ; Vol. 27, No. 15. pp. 2951-2961.
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