TY - JOUR
T1 - Collagen type I containing hybrid hydrogel enhances cardiomyocyte maturation in a 3D cardiac model
AU - Edalat, Sam G.
AU - Jang, Yongjun
AU - Kim, Jongseong
AU - Park, Yongdoo
N1 - Funding Information:
We thank Choi at Korea University for sharing the protocol for the maturation of human embryonic stem cell derived cardiomyocytes and Song at Korea University for helping with the revision process. This study was supported by the grant from the National Research Foundation of Korea, Republic of Korea (Grant No. 2016-M3A9B6947892) and a Korea University Grant.
Funding Information:
Funding: This study was supported by the grant from the National Research Foundation of Korea, Republic of Korea (Grant No. 2016-M3A9B6947892) and a Korea University Grant.
Publisher Copyright:
© 2019 by the authors.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - In vitro maturation of cardiomyocytes in 3D is essential for the development of viable cardiac models for therapeutic and developmental studies. The method by which cardiomyocytes undergoes maturation has significant implications for understanding cardiomyocytes biology. The regulation of the extracellular matrix (ECM) by changing the composition and stiffness is quintessential for engineering a suitable environment for cardiomyocytes maturation. In this paper, we demonstrate that collagen type I, a component of the ECM, plays a crucial role in the maturation of cardiomyocytes. To this end, embryonic stem-cell derived cardiomyocytes were incorporated into Matrigel-based hydrogels with varying collagen type I concentrations of 0 mg, 3 mg, and 6 mg. Each hydrogel was analyzed by measuring the degree of stiffness, the expression levels of MLC2v, TBX18, and pre-miR-21, and the size of the hydrogels. It was shown that among the hydrogel variants, the Matrigel-based hydrogel with 3 mg of collagen type I facilitates cardiomyocyte maturation by increasing MLC2v expression. The treatment of transforming growth factor β1 (TGF-β1) or fibroblast growth factor 4 (FGF-4) on the hydrogels further enhanced the MLC2v expression and thereby cardiomyocyte maturation.
AB - In vitro maturation of cardiomyocytes in 3D is essential for the development of viable cardiac models for therapeutic and developmental studies. The method by which cardiomyocytes undergoes maturation has significant implications for understanding cardiomyocytes biology. The regulation of the extracellular matrix (ECM) by changing the composition and stiffness is quintessential for engineering a suitable environment for cardiomyocytes maturation. In this paper, we demonstrate that collagen type I, a component of the ECM, plays a crucial role in the maturation of cardiomyocytes. To this end, embryonic stem-cell derived cardiomyocytes were incorporated into Matrigel-based hydrogels with varying collagen type I concentrations of 0 mg, 3 mg, and 6 mg. Each hydrogel was analyzed by measuring the degree of stiffness, the expression levels of MLC2v, TBX18, and pre-miR-21, and the size of the hydrogels. It was shown that among the hydrogel variants, the Matrigel-based hydrogel with 3 mg of collagen type I facilitates cardiomyocyte maturation by increasing MLC2v expression. The treatment of transforming growth factor β1 (TGF-β1) or fibroblast growth factor 4 (FGF-4) on the hydrogels further enhanced the MLC2v expression and thereby cardiomyocyte maturation.
KW - Cardiomyocyte maturation
KW - Collagen type I
KW - Embryonic stem-cell
KW - FGF-4
KW - Matrigel-based hydrogel
KW - TGF-β1
UR - http://www.scopus.com/inward/record.url?scp=85065926989&partnerID=8YFLogxK
U2 - 10.3390/polym11040687
DO - 10.3390/polym11040687
M3 - Article
AN - SCOPUS:85065926989
VL - 11
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 4
M1 - 687
ER -