Collagen type I containing hybrid hydrogel enhances cardiomyocyte maturation in a 3D cardiac model

Sam G. Edalat, Yongjun Jang, Jongseong Kim, Yongdoo Park

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

In vitro maturation of cardiomyocytes in 3D is essential for the development of viable cardiac models for therapeutic and developmental studies. The method by which cardiomyocytes undergoes maturation has significant implications for understanding cardiomyocytes biology. The regulation of the extracellular matrix (ECM) by changing the composition and stiffness is quintessential for engineering a suitable environment for cardiomyocytes maturation. In this paper, we demonstrate that collagen type I, a component of the ECM, plays a crucial role in the maturation of cardiomyocytes. To this end, embryonic stem-cell derived cardiomyocytes were incorporated into Matrigel-based hydrogels with varying collagen type I concentrations of 0 mg, 3 mg, and 6 mg. Each hydrogel was analyzed by measuring the degree of stiffness, the expression levels of MLC2v, TBX18, and pre-miR-21, and the size of the hydrogels. It was shown that among the hydrogel variants, the Matrigel-based hydrogel with 3 mg of collagen type I facilitates cardiomyocyte maturation by increasing MLC2v expression. The treatment of transforming growth factor β1 (TGF-β1) or fibroblast growth factor 4 (FGF-4) on the hydrogels further enhanced the MLC2v expression and thereby cardiomyocyte maturation.

Original languageEnglish
Article number687
JournalPolymers
Volume11
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

Keywords

  • Cardiomyocyte maturation
  • Collagen type I
  • Embryonic stem-cell
  • FGF-4
  • Matrigel-based hydrogel
  • TGF-β1

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

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