Collective helicity switching of a DNA-coat assembly

Yongju Kim, Huichang Li, Ying He, Xi Chen, Xiaoteng Ma, Myongsoo Lee

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Hierarchical assemblies of biomolecular subunits can carry out versatile tasks at the cellular level with remarkable spatial and temporal precision 1,2 . As an example, the collective motion and mutual cooperation between complex protein machines mediate essential functions for life, such as replication 3 , synthesis 4 , degradation 5 , repair 6 and transport 7 . Nucleic acid molecules are far less dynamic than proteins and need to bind to specific proteins to form hierarchical structures. The simplest example of these nucleic acid-based structures is provided by a rod-shaped tobacco mosaic virus, which consists of genetic material surrounded by coat proteins 8 . Inspired by the complexity and hierarchical assembly of viruses, a great deal of effort has been devoted to design similarly constructed artificial viruses 9,10 . However, such a wrapping approach makes nucleic acid dynamics insensitive to environmental changes. This limitation generally restricts, for example, the amplification of the conformational dynamics between the right-handed B form to the left-handed Z form of double-stranded deoxyribonucleic acid (DNA) 11,12 . Here we report a virus-like hierarchical assembly in which the native DNA and a synthetic coat undergo repeated collective helicity switching triggered by pH change under physiological conditions. We also show that this collective helicity inversion occurs during translocation of the DNA-coat assembly into intracellular compartments. Translating DNA conformational dynamics into a higher level of hierarchical dynamics may provide an approach to create DNA-based nanomachines.

Original languageEnglish
Pages (from-to)551-556
Number of pages6
JournalNature Nanotechnology
Volume12
Issue number6
DOIs
Publication statusPublished - 2017 Jun 6
Externally publishedYes

Fingerprint

viruses
DNA
Viruses
deoxyribonucleic acid
assembly
Nucleic acids
nucleic acids
Nucleic Acids
proteins
Proteins
Tobacco Mosaic Virus
Virus Assembly
tobacco
translating
Tobacco
Capsid Proteins
compartments
assemblies
Amplification
Repair

ASJC Scopus subject areas

  • Bioengineering
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

Collective helicity switching of a DNA-coat assembly. / Kim, Yongju; Li, Huichang; He, Ying; Chen, Xi; Ma, Xiaoteng; Lee, Myongsoo.

In: Nature Nanotechnology, Vol. 12, No. 6, 06.06.2017, p. 551-556.

Research output: Contribution to journalArticle

Kim, Y, Li, H, He, Y, Chen, X, Ma, X & Lee, M 2017, 'Collective helicity switching of a DNA-coat assembly', Nature Nanotechnology, vol. 12, no. 6, pp. 551-556. https://doi.org/10.1038/nnano.2017.42
Kim, Yongju ; Li, Huichang ; He, Ying ; Chen, Xi ; Ma, Xiaoteng ; Lee, Myongsoo. / Collective helicity switching of a DNA-coat assembly. In: Nature Nanotechnology. 2017 ; Vol. 12, No. 6. pp. 551-556.
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