Abstract
We have developed a highly sensitive and selective colorimetric method for detection of acetylcholine (ACh), using a tandem enzymatic reaction for biological target recognition and silver nanoplates (AgNPLs) for optical signal generation. The ACh molecules are enzymatically hydrolyzed and oxidized into betaine and hydrogen peroxide, the latter of which chemically oxidizes the AgNPLs to generate the "turn-on" signal. To optimize detection sensitivity, the chemical and biological properties of the detection mixtures containing the enzymes, ACh, and AgNPLs were thoroughly investigated with respect to component concentrations and reaction temperatures; a maximum sensitivity of 500 nM for colorimetric detection of ACh was achieved. We further directly compared the signaling profiles of (1) novel nanostructured and (2) conventional molecular chromogens, improving our understanding of the factors that should be considered when designing a detection system.
| Original language | English |
|---|---|
| Pages (from-to) | 7591-7600 |
| Number of pages | 10 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 406 |
| Issue number | 29 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Biosensors
- Enzymes
- Nanoparticles/nanotechnology
- Optical sensors
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
Cite this
Colorimetric detection of acetylcholine with plasmonic nanomaterials signaling. / Oh, Ju Hwan; Kim, Byoung Chan; Lee, Jae-Seung.
In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 29, 2014, p. 7591-7600.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Colorimetric detection of acetylcholine with plasmonic nanomaterials signaling
AU - Oh, Ju Hwan
AU - Kim, Byoung Chan
AU - Lee, Jae-Seung
PY - 2014
Y1 - 2014
N2 - We have developed a highly sensitive and selective colorimetric method for detection of acetylcholine (ACh), using a tandem enzymatic reaction for biological target recognition and silver nanoplates (AgNPLs) for optical signal generation. The ACh molecules are enzymatically hydrolyzed and oxidized into betaine and hydrogen peroxide, the latter of which chemically oxidizes the AgNPLs to generate the "turn-on" signal. To optimize detection sensitivity, the chemical and biological properties of the detection mixtures containing the enzymes, ACh, and AgNPLs were thoroughly investigated with respect to component concentrations and reaction temperatures; a maximum sensitivity of 500 nM for colorimetric detection of ACh was achieved. We further directly compared the signaling profiles of (1) novel nanostructured and (2) conventional molecular chromogens, improving our understanding of the factors that should be considered when designing a detection system.
AB - We have developed a highly sensitive and selective colorimetric method for detection of acetylcholine (ACh), using a tandem enzymatic reaction for biological target recognition and silver nanoplates (AgNPLs) for optical signal generation. The ACh molecules are enzymatically hydrolyzed and oxidized into betaine and hydrogen peroxide, the latter of which chemically oxidizes the AgNPLs to generate the "turn-on" signal. To optimize detection sensitivity, the chemical and biological properties of the detection mixtures containing the enzymes, ACh, and AgNPLs were thoroughly investigated with respect to component concentrations and reaction temperatures; a maximum sensitivity of 500 nM for colorimetric detection of ACh was achieved. We further directly compared the signaling profiles of (1) novel nanostructured and (2) conventional molecular chromogens, improving our understanding of the factors that should be considered when designing a detection system.
KW - Biosensors
KW - Enzymes
KW - Nanoparticles/nanotechnology
KW - Optical sensors
UR - http://www.scopus.com/inward/record.url?scp=84925541578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84925541578&partnerID=8YFLogxK
U2 - 10.1007/s00216-014-8199-4
DO - 10.1007/s00216-014-8199-4
M3 - Article
VL - 406
SP - 7591
EP - 7600
JO - Fresenius Zeitschrift fur Analytische Chemie
JF - Fresenius Zeitschrift fur Analytische Chemie
SN - 0016-1152
IS - 29
ER -