Colorimetric pH measurement of animal cell culture media

Juno Jang, Soo Jin Moon, Sung Hwan Hong, Ik Hwan Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Most animal cell culture media can be buffered using bicarbonate and high pressure CO2 in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO2 pressure between the culture media and the atmosphere. Therefore, it is important to measure the exact pH of the culture media in an intact closed system. In this study, a pH measurement method was developed using visible light. The pH was calculated from light absorbance by the cells and by the culture media. This method was successfully applied to both suspension and anchorage-dependent cell cultures.

Original languageEnglish
Pages (from-to)1599-1607
Number of pages9
JournalBiotechnology letters
Volume32
Issue number11
DOIs
Publication statusPublished - 2010

Keywords

  • Animal cell culture
  • Process analytical technology
  • Process monitoring
  • Visible light
  • pH measurement

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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