Colorimetric SNP Genotyping Based on Allele-Specific PCR by Using a Thiol-Labeled Primer

Ye Lim Jung, Cheulhee Jung, Harshala Parab, Dae Yeon Cho, Hyun Gyu Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Red gold/blue gold: A highly convenient colorimetric method for the identification of single-nucleotide polymorphism was developed by utilizing gold nanoparticles and an allele-specific polymerase chain reaction. Its diagnostic capability was successfully demonstrated by correctly identifying various mutations in the BRCA1 gene.

Original languageEnglish
Pages (from-to)1387-1390
Number of pages4
JournalChemBioChem
Volume12
Issue number9
DOIs
Publication statusPublished - 2011 Jun 14
Externally publishedYes

Fingerprint

Sulfhydryl Compounds
Gold
Single Nucleotide Polymorphism
Alleles
Polymerase Chain Reaction
BRCA1 Gene
Polymerase chain reaction
Polymorphism
Nanoparticles
Nucleotides
Genes
Mutation

Keywords

  • BRCA
  • Gold
  • Nanoparticles
  • Polymerase chain reaction
  • Single nucleotide polymorphism
  • Thiolated primer

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology

Cite this

Colorimetric SNP Genotyping Based on Allele-Specific PCR by Using a Thiol-Labeled Primer. / Jung, Ye Lim; Jung, Cheulhee; Parab, Harshala; Cho, Dae Yeon; Park, Hyun Gyu.

In: ChemBioChem, Vol. 12, No. 9, 14.06.2011, p. 1387-1390.

Research output: Contribution to journalArticle

Jung, Ye Lim ; Jung, Cheulhee ; Parab, Harshala ; Cho, Dae Yeon ; Park, Hyun Gyu. / Colorimetric SNP Genotyping Based on Allele-Specific PCR by Using a Thiol-Labeled Primer. In: ChemBioChem. 2011 ; Vol. 12, No. 9. pp. 1387-1390.
@article{4e90b52927364d08bfdbd9459770372c,
title = "Colorimetric SNP Genotyping Based on Allele-Specific PCR by Using a Thiol-Labeled Primer",
abstract = "Red gold/blue gold: A highly convenient colorimetric method for the identification of single-nucleotide polymorphism was developed by utilizing gold nanoparticles and an allele-specific polymerase chain reaction. Its diagnostic capability was successfully demonstrated by correctly identifying various mutations in the BRCA1 gene.",
keywords = "BRCA, Gold, Nanoparticles, Polymerase chain reaction, Single nucleotide polymorphism, Thiolated primer",
author = "Jung, {Ye Lim} and Cheulhee Jung and Harshala Parab and Cho, {Dae Yeon} and Park, {Hyun Gyu}",
year = "2011",
month = "6",
day = "14",
doi = "10.1002/cbic.201100098",
language = "English",
volume = "12",
pages = "1387--1390",
journal = "ChemBioChem",
issn = "1439-4227",
publisher = "Wiley-VCH Verlag",
number = "9",

}

TY - JOUR

T1 - Colorimetric SNP Genotyping Based on Allele-Specific PCR by Using a Thiol-Labeled Primer

AU - Jung, Ye Lim

AU - Jung, Cheulhee

AU - Parab, Harshala

AU - Cho, Dae Yeon

AU - Park, Hyun Gyu

PY - 2011/6/14

Y1 - 2011/6/14

N2 - Red gold/blue gold: A highly convenient colorimetric method for the identification of single-nucleotide polymorphism was developed by utilizing gold nanoparticles and an allele-specific polymerase chain reaction. Its diagnostic capability was successfully demonstrated by correctly identifying various mutations in the BRCA1 gene.

AB - Red gold/blue gold: A highly convenient colorimetric method for the identification of single-nucleotide polymorphism was developed by utilizing gold nanoparticles and an allele-specific polymerase chain reaction. Its diagnostic capability was successfully demonstrated by correctly identifying various mutations in the BRCA1 gene.

KW - BRCA

KW - Gold

KW - Nanoparticles

KW - Polymerase chain reaction

KW - Single nucleotide polymorphism

KW - Thiolated primer

UR - http://www.scopus.com/inward/record.url?scp=79958715238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958715238&partnerID=8YFLogxK

U2 - 10.1002/cbic.201100098

DO - 10.1002/cbic.201100098

M3 - Article

VL - 12

SP - 1387

EP - 1390

JO - ChemBioChem

JF - ChemBioChem

SN - 1439-4227

IS - 9

ER -