Combinations of growth factors enhance the potency of islets in vitro

Jong Yeon Lim, Byoung Hoon Min, Byoung Geun Kim, Jun Seop Shin, Chang Sook Park, Tai Wook Yoon, Sung Sik Han

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

OBJECTIVES: Recent studies have demonstrated the impressive expansion of β cells in vitro. But unfortunately, expanded β cells do not function in the same way as fully differentiated β cells. Therefore, we developed a condition that would allow islet cells to proliferate while maintaining their endocrine function. METHODS: We tested the different use of growth factors in a different culture period. And we tested the possibility of adult islets, which expanded during a short period, as a clinical source of islet cells by comparing the efficiency of transplantation of cultured islets with that of fresh islets. RESULTS: The islets showed a time-dependent increase in proliferative activity, reaching 32.2% on day 5. After 5 days of culture, the efficiency of transplantation of cultured islets was increased (2-fold) in comparison to that of noncultured islets. Moreover, islet transplantation immediately induced normoglycemia at a level equal to native islets. CONCLUSIONS: These findings suggest that adult β cells have the potential to proliferate while maintaining their endocrine function, which can be improved through careful regulation of proliferation and differentiation.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalPancreas
Volume38
Issue number4
DOIs
Publication statusPublished - 2009 May

Keywords

  • Differentiation
  • Growth factor
  • Islet
  • Proliferation
  • Transplantation

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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  • Cite this

    Lim, J. Y., Min, B. H., Kim, B. G., Shin, J. S., Park, C. S., Yoon, T. W., & Han, S. S. (2009). Combinations of growth factors enhance the potency of islets in vitro. Pancreas, 38(4), 447-453. https://doi.org/10.1097/MPA.0b013e318197a62e