Combined effect of diet and cervical microbiome on the risk of cervical intraepithelial neoplasia

Sang Soo Seo, Hea Young Oh, Jae Kwan Lee, Ji Sook Kong, Dong Ock Lee, Mi Kyung Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background & aims Several food groups or dietary factors and the cervical microbiota may be involved in cervical carcinogenesis, but the evidence is not clear yet. We aimed to assess the association between dietary pattern and cervical intraepithelial neoplasia (CIN) and the combined effect of dietary pattern and cervical microbiome on the risk of CIN. Methods The cervical microbiota and diet assessed by pyrosequencing and a food-frequency questionnaire, respectively, of 65 women with CIN and 72 control women were used in this study. Principal component analysis and cluster analysis were used to identify dietary patterns and microbiome community types, respectively. The association between dietary pattern and CIN risk was assessed using multivariable logistic regression analysis. The combined effect of dietary pattern and microbiome on CIN risk was determined using relative excess risk due to interaction (RERI) and synergy index (S). Results Two dietary patterns and four community types were identified: prudent diet characterized by higher intake of vegetables and fishes; semi-Western diet characterized by higher intake of bread, dairy products, eggs, and soft drinks and relatively higher fat intake ratio; and Lactobacillus crispatus–, L. iners–, Atopobium vaginae–, and Prevotella bivia–dominant types. The high-scoring group of participants with a semi-Western diet had a higher risk of CIN (odds ratio [OR] 3.44, 95% confidence interval [CI] 1.11–10.7, p = 0.03), compared with the low or medium-scoring group of those with a semi-Western diet. L. iners–dominant (OR 6.39, 95% CI 1.52–26.7, p = 0.01) and A. vaginae–dominant (OR 4.99, 95% CI 1.17–21.3, p = 0.03) dominant types had a higher risk of CIN, compared with the L. crispatus–dominant type. The synergistic effect of semi-Western diet and A. vaginae–dominant type on CIN risk was observed (OR 20.8, 95% CI 2.21–195.6, p = 0.01, RERI/S 9.64/1.96). Conclusions Our findings suggest that semi-Western diet and its combination with A. vaginae–dominant microflora may represent an important risk factor for cervical neoplasia.

Original languageEnglish
Pages (from-to)1434-1441
Number of pages8
JournalClinical Nutrition
Volume35
Issue number6
DOIs
Publication statusPublished - 2016 Dec 1

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Cervical Intraepithelial Neoplasia
Microbiota
Diet
Odds Ratio
Confidence Intervals
Prevotella
Carbonated Beverages
Food
Dairy Products
Bread
Vagina
Principal Component Analysis
Vegetables
Eggs
Cluster Analysis
Carcinogenesis
Fishes
Logistic Models
Fats
Regression Analysis

Keywords

  • Cervical intraepithelial neoplasia
  • Dietary pattern
  • Microbiome
  • Pyrosequencing
  • South Korea

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Critical Care and Intensive Care Medicine

Cite this

Combined effect of diet and cervical microbiome on the risk of cervical intraepithelial neoplasia. / Seo, Sang Soo; Oh, Hea Young; Lee, Jae Kwan; Kong, Ji Sook; Lee, Dong Ock; Kim, Mi Kyung.

In: Clinical Nutrition, Vol. 35, No. 6, 01.12.2016, p. 1434-1441.

Research output: Contribution to journalArticle

Seo, Sang Soo ; Oh, Hea Young ; Lee, Jae Kwan ; Kong, Ji Sook ; Lee, Dong Ock ; Kim, Mi Kyung. / Combined effect of diet and cervical microbiome on the risk of cervical intraepithelial neoplasia. In: Clinical Nutrition. 2016 ; Vol. 35, No. 6. pp. 1434-1441.
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abstract = "Background & aims Several food groups or dietary factors and the cervical microbiota may be involved in cervical carcinogenesis, but the evidence is not clear yet. We aimed to assess the association between dietary pattern and cervical intraepithelial neoplasia (CIN) and the combined effect of dietary pattern and cervical microbiome on the risk of CIN. Methods The cervical microbiota and diet assessed by pyrosequencing and a food-frequency questionnaire, respectively, of 65 women with CIN and 72 control women were used in this study. Principal component analysis and cluster analysis were used to identify dietary patterns and microbiome community types, respectively. The association between dietary pattern and CIN risk was assessed using multivariable logistic regression analysis. The combined effect of dietary pattern and microbiome on CIN risk was determined using relative excess risk due to interaction (RERI) and synergy index (S). Results Two dietary patterns and four community types were identified: prudent diet characterized by higher intake of vegetables and fishes; semi-Western diet characterized by higher intake of bread, dairy products, eggs, and soft drinks and relatively higher fat intake ratio; and Lactobacillus crispatus–, L. iners–, Atopobium vaginae–, and Prevotella bivia–dominant types. The high-scoring group of participants with a semi-Western diet had a higher risk of CIN (odds ratio [OR] 3.44, 95{\%} confidence interval [CI] 1.11–10.7, p = 0.03), compared with the low or medium-scoring group of those with a semi-Western diet. L. iners–dominant (OR 6.39, 95{\%} CI 1.52–26.7, p = 0.01) and A. vaginae–dominant (OR 4.99, 95{\%} CI 1.17–21.3, p = 0.03) dominant types had a higher risk of CIN, compared with the L. crispatus–dominant type. The synergistic effect of semi-Western diet and A. vaginae–dominant type on CIN risk was observed (OR 20.8, 95{\%} CI 2.21–195.6, p = 0.01, RERI/S 9.64/1.96). Conclusions Our findings suggest that semi-Western diet and its combination with A. vaginae–dominant microflora may represent an important risk factor for cervical neoplasia.",
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AU - Kong, Ji Sook

AU - Lee, Dong Ock

AU - Kim, Mi Kyung

PY - 2016/12/1

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N2 - Background & aims Several food groups or dietary factors and the cervical microbiota may be involved in cervical carcinogenesis, but the evidence is not clear yet. We aimed to assess the association between dietary pattern and cervical intraepithelial neoplasia (CIN) and the combined effect of dietary pattern and cervical microbiome on the risk of CIN. Methods The cervical microbiota and diet assessed by pyrosequencing and a food-frequency questionnaire, respectively, of 65 women with CIN and 72 control women were used in this study. Principal component analysis and cluster analysis were used to identify dietary patterns and microbiome community types, respectively. The association between dietary pattern and CIN risk was assessed using multivariable logistic regression analysis. The combined effect of dietary pattern and microbiome on CIN risk was determined using relative excess risk due to interaction (RERI) and synergy index (S). Results Two dietary patterns and four community types were identified: prudent diet characterized by higher intake of vegetables and fishes; semi-Western diet characterized by higher intake of bread, dairy products, eggs, and soft drinks and relatively higher fat intake ratio; and Lactobacillus crispatus–, L. iners–, Atopobium vaginae–, and Prevotella bivia–dominant types. The high-scoring group of participants with a semi-Western diet had a higher risk of CIN (odds ratio [OR] 3.44, 95% confidence interval [CI] 1.11–10.7, p = 0.03), compared with the low or medium-scoring group of those with a semi-Western diet. L. iners–dominant (OR 6.39, 95% CI 1.52–26.7, p = 0.01) and A. vaginae–dominant (OR 4.99, 95% CI 1.17–21.3, p = 0.03) dominant types had a higher risk of CIN, compared with the L. crispatus–dominant type. The synergistic effect of semi-Western diet and A. vaginae–dominant type on CIN risk was observed (OR 20.8, 95% CI 2.21–195.6, p = 0.01, RERI/S 9.64/1.96). Conclusions Our findings suggest that semi-Western diet and its combination with A. vaginae–dominant microflora may represent an important risk factor for cervical neoplasia.

AB - Background & aims Several food groups or dietary factors and the cervical microbiota may be involved in cervical carcinogenesis, but the evidence is not clear yet. We aimed to assess the association between dietary pattern and cervical intraepithelial neoplasia (CIN) and the combined effect of dietary pattern and cervical microbiome on the risk of CIN. Methods The cervical microbiota and diet assessed by pyrosequencing and a food-frequency questionnaire, respectively, of 65 women with CIN and 72 control women were used in this study. Principal component analysis and cluster analysis were used to identify dietary patterns and microbiome community types, respectively. The association between dietary pattern and CIN risk was assessed using multivariable logistic regression analysis. The combined effect of dietary pattern and microbiome on CIN risk was determined using relative excess risk due to interaction (RERI) and synergy index (S). Results Two dietary patterns and four community types were identified: prudent diet characterized by higher intake of vegetables and fishes; semi-Western diet characterized by higher intake of bread, dairy products, eggs, and soft drinks and relatively higher fat intake ratio; and Lactobacillus crispatus–, L. iners–, Atopobium vaginae–, and Prevotella bivia–dominant types. The high-scoring group of participants with a semi-Western diet had a higher risk of CIN (odds ratio [OR] 3.44, 95% confidence interval [CI] 1.11–10.7, p = 0.03), compared with the low or medium-scoring group of those with a semi-Western diet. L. iners–dominant (OR 6.39, 95% CI 1.52–26.7, p = 0.01) and A. vaginae–dominant (OR 4.99, 95% CI 1.17–21.3, p = 0.03) dominant types had a higher risk of CIN, compared with the L. crispatus–dominant type. The synergistic effect of semi-Western diet and A. vaginae–dominant type on CIN risk was observed (OR 20.8, 95% CI 2.21–195.6, p = 0.01, RERI/S 9.64/1.96). Conclusions Our findings suggest that semi-Western diet and its combination with A. vaginae–dominant microflora may represent an important risk factor for cervical neoplasia.

KW - Cervical intraepithelial neoplasia

KW - Dietary pattern

KW - Microbiome

KW - Pyrosequencing

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