Combined effects of plant extracts in inhibiting the growth of Bacillus cereus in reconstituted infant rice cereal

Hyejung Jun, Jinsol Kim, Jihyun Bang, Hoikyung Kim, Larry R. Beuchat, Jee-Hoon Ryu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A study was done to determine the potential use of plant extracts to inhibit the growth of Bacillus cereus in reconstituted infant rice cereal. A total of 2116 extracts were screened for inhibitory activity against B. cereus using an agar well diffusion assay. The minimal inhibitory concentrations (MIC) and minimal lethal concentrations (MLC) of 14 promising extracts in tryptic soy broth (TSB) were determined. Dryopteris erythrosora (autumn fern) root extract showed the lowest MIC (0.0156. mg/ml), followed by Siegesbeckia glabrescens (Siegesbeckia herb) leaf (0.0313. mg/ml), Morus alba (white mulberry) cortex (0.0313. mg/ml), Carex pumila (sand sedge) root (0.0625. mg/ml), and Citrus paradisi (grapefruit) seed (0.0625. mg/ml) extracts. The order of MLCs of extracts was D. erythrosora root (0.0156. mg/ml) < S. glabrescens leaf (0.0313. mg/ml) < M. alba cortex (0.0625. mg/ml) = Siegesbeckia pubescens aerial part (0.0625. mg/ml) < C. paradisi seed (0.1250. mg/ml). The combined inhibitory effects of extracts against B. cereus in TSB were determined using a checkerboard assay. A combination of D. erythrosora and C. pumila extracts showed a partial synergistic inhibition, with a fractional inhibitory concentration index (FICI) of 0.75. Single and combined inhibitory activities of selected plant extracts against B. cereus in reconstituted infant rice cereal were investigated. The MICs of S. glabrescens, M. alba, D. erythrosora, and C. pumila extracts against B. cereus were 1.0, 2.0, 2.0, and 8.0. mg/ml, respectively. A combination of D. erythrosora (1.00. mg/ml) and C. pumila (1.00. mg/ml) extracts showed a partial synergistic effect (FICI 0.63) in inhibiting the growth of B. cereus. Results indicate that by combining extracts, the amounts of D. erythrosora and C. pumila extracts can be reduced by 50% and 87.5%, respectively, compared with individual extracts, and give similar inhibitory activity in reconstituted infant rice cereal. Sensory evaluation showed that supplementing reconstituted infant rice cereal with plant extracts reduces sensorial quality. These observations will be useful when developing and applying interventions using natural plant extracts to inhibit B. cereus in foods.

Original languageEnglish
Pages (from-to)260-266
Number of pages7
JournalInternational Journal of Food Microbiology
Volume160
Issue number3
DOIs
Publication statusPublished - 2013 Jan 1

Fingerprint

Plant extracts
Bacillus cereus
Plant Extracts
plant extracts
Dryopteris erythrosora
rice
Citrus paradisi
extracts
Growth
Sigesbeckia
Morus alba
Morus
Seed
Assays
Seeds
Dryopteris
Ferns
Oryza
Edible Grain
cortex

Keywords

  • Bacillus cereus
  • Infant rice cereal
  • Naturally occurring antimicrobial agents

ASJC Scopus subject areas

  • Food Science
  • Microbiology
  • Safety, Risk, Reliability and Quality

Cite this

Combined effects of plant extracts in inhibiting the growth of Bacillus cereus in reconstituted infant rice cereal. / Jun, Hyejung; Kim, Jinsol; Bang, Jihyun; Kim, Hoikyung; Beuchat, Larry R.; Ryu, Jee-Hoon.

In: International Journal of Food Microbiology, Vol. 160, No. 3, 01.01.2013, p. 260-266.

Research output: Contribution to journalArticle

Jun, Hyejung ; Kim, Jinsol ; Bang, Jihyun ; Kim, Hoikyung ; Beuchat, Larry R. ; Ryu, Jee-Hoon. / Combined effects of plant extracts in inhibiting the growth of Bacillus cereus in reconstituted infant rice cereal. In: International Journal of Food Microbiology. 2013 ; Vol. 160, No. 3. pp. 260-266.
@article{d84190fb637a4755b3949a87d6ac2e6f,
title = "Combined effects of plant extracts in inhibiting the growth of Bacillus cereus in reconstituted infant rice cereal",
abstract = "A study was done to determine the potential use of plant extracts to inhibit the growth of Bacillus cereus in reconstituted infant rice cereal. A total of 2116 extracts were screened for inhibitory activity against B. cereus using an agar well diffusion assay. The minimal inhibitory concentrations (MIC) and minimal lethal concentrations (MLC) of 14 promising extracts in tryptic soy broth (TSB) were determined. Dryopteris erythrosora (autumn fern) root extract showed the lowest MIC (0.0156. mg/ml), followed by Siegesbeckia glabrescens (Siegesbeckia herb) leaf (0.0313. mg/ml), Morus alba (white mulberry) cortex (0.0313. mg/ml), Carex pumila (sand sedge) root (0.0625. mg/ml), and Citrus paradisi (grapefruit) seed (0.0625. mg/ml) extracts. The order of MLCs of extracts was D. erythrosora root (0.0156. mg/ml) < S. glabrescens leaf (0.0313. mg/ml) < M. alba cortex (0.0625. mg/ml) = Siegesbeckia pubescens aerial part (0.0625. mg/ml) < C. paradisi seed (0.1250. mg/ml). The combined inhibitory effects of extracts against B. cereus in TSB were determined using a checkerboard assay. A combination of D. erythrosora and C. pumila extracts showed a partial synergistic inhibition, with a fractional inhibitory concentration index (FICI) of 0.75. Single and combined inhibitory activities of selected plant extracts against B. cereus in reconstituted infant rice cereal were investigated. The MICs of S. glabrescens, M. alba, D. erythrosora, and C. pumila extracts against B. cereus were 1.0, 2.0, 2.0, and 8.0. mg/ml, respectively. A combination of D. erythrosora (1.00. mg/ml) and C. pumila (1.00. mg/ml) extracts showed a partial synergistic effect (FICI 0.63) in inhibiting the growth of B. cereus. Results indicate that by combining extracts, the amounts of D. erythrosora and C. pumila extracts can be reduced by 50{\%} and 87.5{\%}, respectively, compared with individual extracts, and give similar inhibitory activity in reconstituted infant rice cereal. Sensory evaluation showed that supplementing reconstituted infant rice cereal with plant extracts reduces sensorial quality. These observations will be useful when developing and applying interventions using natural plant extracts to inhibit B. cereus in foods.",
keywords = "Bacillus cereus, Infant rice cereal, Naturally occurring antimicrobial agents",
author = "Hyejung Jun and Jinsol Kim and Jihyun Bang and Hoikyung Kim and Beuchat, {Larry R.} and Jee-Hoon Ryu",
year = "2013",
month = "1",
day = "1",
doi = "10.1016/j.ijfoodmicro.2012.10.020",
language = "English",
volume = "160",
pages = "260--266",
journal = "International Journal of Food Microbiology",
issn = "0168-1605",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Combined effects of plant extracts in inhibiting the growth of Bacillus cereus in reconstituted infant rice cereal

AU - Jun, Hyejung

AU - Kim, Jinsol

AU - Bang, Jihyun

AU - Kim, Hoikyung

AU - Beuchat, Larry R.

AU - Ryu, Jee-Hoon

PY - 2013/1/1

Y1 - 2013/1/1

N2 - A study was done to determine the potential use of plant extracts to inhibit the growth of Bacillus cereus in reconstituted infant rice cereal. A total of 2116 extracts were screened for inhibitory activity against B. cereus using an agar well diffusion assay. The minimal inhibitory concentrations (MIC) and minimal lethal concentrations (MLC) of 14 promising extracts in tryptic soy broth (TSB) were determined. Dryopteris erythrosora (autumn fern) root extract showed the lowest MIC (0.0156. mg/ml), followed by Siegesbeckia glabrescens (Siegesbeckia herb) leaf (0.0313. mg/ml), Morus alba (white mulberry) cortex (0.0313. mg/ml), Carex pumila (sand sedge) root (0.0625. mg/ml), and Citrus paradisi (grapefruit) seed (0.0625. mg/ml) extracts. The order of MLCs of extracts was D. erythrosora root (0.0156. mg/ml) < S. glabrescens leaf (0.0313. mg/ml) < M. alba cortex (0.0625. mg/ml) = Siegesbeckia pubescens aerial part (0.0625. mg/ml) < C. paradisi seed (0.1250. mg/ml). The combined inhibitory effects of extracts against B. cereus in TSB were determined using a checkerboard assay. A combination of D. erythrosora and C. pumila extracts showed a partial synergistic inhibition, with a fractional inhibitory concentration index (FICI) of 0.75. Single and combined inhibitory activities of selected plant extracts against B. cereus in reconstituted infant rice cereal were investigated. The MICs of S. glabrescens, M. alba, D. erythrosora, and C. pumila extracts against B. cereus were 1.0, 2.0, 2.0, and 8.0. mg/ml, respectively. A combination of D. erythrosora (1.00. mg/ml) and C. pumila (1.00. mg/ml) extracts showed a partial synergistic effect (FICI 0.63) in inhibiting the growth of B. cereus. Results indicate that by combining extracts, the amounts of D. erythrosora and C. pumila extracts can be reduced by 50% and 87.5%, respectively, compared with individual extracts, and give similar inhibitory activity in reconstituted infant rice cereal. Sensory evaluation showed that supplementing reconstituted infant rice cereal with plant extracts reduces sensorial quality. These observations will be useful when developing and applying interventions using natural plant extracts to inhibit B. cereus in foods.

AB - A study was done to determine the potential use of plant extracts to inhibit the growth of Bacillus cereus in reconstituted infant rice cereal. A total of 2116 extracts were screened for inhibitory activity against B. cereus using an agar well diffusion assay. The minimal inhibitory concentrations (MIC) and minimal lethal concentrations (MLC) of 14 promising extracts in tryptic soy broth (TSB) were determined. Dryopteris erythrosora (autumn fern) root extract showed the lowest MIC (0.0156. mg/ml), followed by Siegesbeckia glabrescens (Siegesbeckia herb) leaf (0.0313. mg/ml), Morus alba (white mulberry) cortex (0.0313. mg/ml), Carex pumila (sand sedge) root (0.0625. mg/ml), and Citrus paradisi (grapefruit) seed (0.0625. mg/ml) extracts. The order of MLCs of extracts was D. erythrosora root (0.0156. mg/ml) < S. glabrescens leaf (0.0313. mg/ml) < M. alba cortex (0.0625. mg/ml) = Siegesbeckia pubescens aerial part (0.0625. mg/ml) < C. paradisi seed (0.1250. mg/ml). The combined inhibitory effects of extracts against B. cereus in TSB were determined using a checkerboard assay. A combination of D. erythrosora and C. pumila extracts showed a partial synergistic inhibition, with a fractional inhibitory concentration index (FICI) of 0.75. Single and combined inhibitory activities of selected plant extracts against B. cereus in reconstituted infant rice cereal were investigated. The MICs of S. glabrescens, M. alba, D. erythrosora, and C. pumila extracts against B. cereus were 1.0, 2.0, 2.0, and 8.0. mg/ml, respectively. A combination of D. erythrosora (1.00. mg/ml) and C. pumila (1.00. mg/ml) extracts showed a partial synergistic effect (FICI 0.63) in inhibiting the growth of B. cereus. Results indicate that by combining extracts, the amounts of D. erythrosora and C. pumila extracts can be reduced by 50% and 87.5%, respectively, compared with individual extracts, and give similar inhibitory activity in reconstituted infant rice cereal. Sensory evaluation showed that supplementing reconstituted infant rice cereal with plant extracts reduces sensorial quality. These observations will be useful when developing and applying interventions using natural plant extracts to inhibit B. cereus in foods.

KW - Bacillus cereus

KW - Infant rice cereal

KW - Naturally occurring antimicrobial agents

UR - http://www.scopus.com/inward/record.url?scp=84871975734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871975734&partnerID=8YFLogxK

U2 - 10.1016/j.ijfoodmicro.2012.10.020

DO - 10.1016/j.ijfoodmicro.2012.10.020

M3 - Article

C2 - 23290233

AN - SCOPUS:84871975734

VL - 160

SP - 260

EP - 266

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

IS - 3

ER -