Combined Positron Emission Tomography and Cerenkov Luminescence Imaging of Sentinel Lymph Nodes Using PEGylated Radionuclide-Embedded Gold Nanoparticles

Sang Bong Lee, Ghil Suk Yoon, Sang Woo Lee, Shin Young Jeong, Byeong Cheol Ahn, Dong-Kwon Lim, Jaetae Lee, Yong Hyun Jeon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

New imaging probes with high sensitivity and stability are urgently needed to accurately detect sentinel lymph nodes (SLNs) for successful cancer diagnosis. Herein, the use of highly sensitive and stable PEGylated radionuclide-embedded gold nanoparticles (PEG-RIe-AuNPs) is reported for the detection of SLNs by combined positron emission tomography and Cerenkov luminescence imaging (PET/CLI). PEG-RIe-AuNPs show high sensitivity and stability both in vitro and in vivo, and are not toxic to normal ovarian and immune cells. In vivo PET/CLI imaging clearly reveals SLNs as early as 1 h post PEG-RIe-AuNP-injection, with peak signals achieved at 6 h postinjection, which is consistent with the biodistribution results. Taken together, the data provide strong evidence that PEG-RIe-AuNPs are promising as potential lymphatic tracers in biomedical imaging for pre and intraoperative surgical guidance.

Original languageEnglish
Pages (from-to)4894-4901
Number of pages8
JournalSmall
Volume12
Issue number35
DOIs
Publication statusPublished - 2016 Sep 21

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Keywords

  • cerenkov luminescence imaging
  • gold nanoparticles
  • positron emission tomography
  • sentinel lymph node

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

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