Comparative analysis of 5,211 leaf ESTs of wild rice (Oryza minuta)

S. K. Cho, S. H. Ok, J. U. Jeung, K. S. Shim, K. W. Jung, M. K. You, K. H. Kang, Y. S. Chung, H. C. Choi, H. P. Moon, Jeong Sheop Shin

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Abstract

The expressed sequence tags (ESTs) presented in this report are the first transcriptomes of wild rice. A cDNA library was constructed from 4-week-old leaf samples of greenhouse-grown Oryza minuta. The 5,211 cDNA clones of O. minuta represent 3,401 unique sequences, consisting of 2,787 singletons and 614 assembled sequences. Database comparisons of the cDNAs in GenBank's non-redundant databases using BLAST revealed that 4,957 of the 5,211 cDNAs (95.1%) showed a high degree of sequence homology to genes from other organisms. Most of the transcripts identified were genes related to metabolism, energy, protein biosynthesis and subcellular localization. The metabolism and energy categories of the O. minuta ESTs showed a considerably higher gene expression level than those of O. sativa ESTs. These data and genes can be utilized in rice breeding.

Original languageEnglish
Pages (from-to)839-847
Number of pages9
JournalPlant Cell Reports
Volume22
Issue number11
DOIs
Publication statusPublished - 2004 Jun 1

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Keywords

  • Expressed sequence tag
  • Functional classification
  • Oryza minuta
  • Vegetative stage
  • Wild rice

ASJC Scopus subject areas

  • Plant Science

Cite this

Cho, S. K., Ok, S. H., Jeung, J. U., Shim, K. S., Jung, K. W., You, M. K., Kang, K. H., Chung, Y. S., Choi, H. C., Moon, H. P., & Shin, J. S. (2004). Comparative analysis of 5,211 leaf ESTs of wild rice (Oryza minuta). Plant Cell Reports, 22(11), 839-847. https://doi.org/10.1007/s00299-004-0764-4