Comparative analysis of serum proteomes of patients with cardiovascular disease

Hyun Jung Kim, Hyun Syuk Yoo, Pan Kyeom Kim, Mi Ryung Kim, Hyup Woo Lee, Chan Wha Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalClinical Biochemistry
Volume44
Issue number2-3
DOIs
Publication statusPublished - 2011 Feb 1

Fingerprint

Proteome
Retinol-Binding Proteins
Cardiovascular Diseases
Serum
Ceruloplasmin
Healthy Volunteers
Western Blotting
Hemopexin
Proteins
Blood vessels
Biomarkers
Blood Vessels
Blood Proteins
Deposits
Gels
Enzyme-Linked Immunosorbent Assay

Keywords

  • Biomarkers
  • Cardiovascular disease
  • Electrospray ionization quadrupole time-of-flight
  • Proteome
  • RBP4
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Comparative analysis of serum proteomes of patients with cardiovascular disease. / Kim, Hyun Jung; Yoo, Hyun Syuk; Kim, Pan Kyeom; Kim, Mi Ryung; Lee, Hyup Woo; Kim, Chan Wha.

In: Clinical Biochemistry, Vol. 44, No. 2-3, 01.02.2011, p. 178-184.

Research output: Contribution to journalArticle

Kim, Hyun Jung ; Yoo, Hyun Syuk ; Kim, Pan Kyeom ; Kim, Mi Ryung ; Lee, Hyup Woo ; Kim, Chan Wha. / Comparative analysis of serum proteomes of patients with cardiovascular disease. In: Clinical Biochemistry. 2011 ; Vol. 44, No. 2-3. pp. 178-184.
@article{4b37a162555e43e5ad977e884c9ea100,
title = "Comparative analysis of serum proteomes of patients with cardiovascular disease",
abstract = "Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.",
keywords = "Biomarkers, Cardiovascular disease, Electrospray ionization quadrupole time-of-flight, Proteome, RBP4, Two-dimensional gel electrophoresis",
author = "Kim, {Hyun Jung} and Yoo, {Hyun Syuk} and Kim, {Pan Kyeom} and Kim, {Mi Ryung} and Lee, {Hyup Woo} and Kim, {Chan Wha}",
year = "2011",
month = "2",
day = "1",
doi = "10.1016/j.clinbiochem.2010.09.027",
language = "English",
volume = "44",
pages = "178--184",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier Inc.",
number = "2-3",

}

TY - JOUR

T1 - Comparative analysis of serum proteomes of patients with cardiovascular disease

AU - Kim, Hyun Jung

AU - Yoo, Hyun Syuk

AU - Kim, Pan Kyeom

AU - Kim, Mi Ryung

AU - Lee, Hyup Woo

AU - Kim, Chan Wha

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.

AB - Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.

KW - Biomarkers

KW - Cardiovascular disease

KW - Electrospray ionization quadrupole time-of-flight

KW - Proteome

KW - RBP4

KW - Two-dimensional gel electrophoresis

UR - http://www.scopus.com/inward/record.url?scp=79151472174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79151472174&partnerID=8YFLogxK

U2 - 10.1016/j.clinbiochem.2010.09.027

DO - 10.1016/j.clinbiochem.2010.09.027

M3 - Article

C2 - 20934420

AN - SCOPUS:79151472174

VL - 44

SP - 178

EP - 184

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

IS - 2-3

ER -