Comparative analysis of serum proteomes of patients with cardiovascular disease

Hyun Jung Kim; Hyun Syuk Yoo; Pan Kyeom Kim; Mi Ryung Kim; Hyup Woo Lee; Chan Wha Kim

doi:10.1016/j.clinbiochem.2010.09.027

Comparative analysis of serum proteomes of patients with cardiovascular disease

Hyun Jung Kim, Hyun Syuk Yoo, Pan Kyeom Kim, Mi Ryung Kim, Hyup Woo Lee, Chan Wha Kim

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.

Original language	English
Pages (from-to)	178-184
Number of pages	7
Journal	Clinical Biochemistry
Volume	44
Issue number	2-3
DOIs	https://doi.org/10.1016/j.clinbiochem.2010.09.027
Publication status	Published - 2011 Feb

Keywords

Biomarkers
Cardiovascular disease
Electrospray ionization quadrupole time-of-flight
Proteome
RBP4
Two-dimensional gel electrophoresis

ASJC Scopus subject areas

Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.clinbiochem.2010.09.027

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title = "Comparative analysis of serum proteomes of patients with cardiovascular disease",

abstract = "Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.",

keywords = "Biomarkers, Cardiovascular disease, Electrospray ionization quadrupole time-of-flight, Proteome, RBP4, Two-dimensional gel electrophoresis",

author = "Kim, {Hyun Jung} and Yoo, {Hyun Syuk} and Kim, {Pan Kyeom} and Kim, {Mi Ryung} and Lee, {Hyup Woo} and Kim, {Chan Wha}",

year = "2011",

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doi = "10.1016/j.clinbiochem.2010.09.027",

language = "English",

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pages = "178--184",

journal = "Clinical Biochemistry",

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T1 - Comparative analysis of serum proteomes of patients with cardiovascular disease

AU - Kim, Hyun Jung

AU - Yoo, Hyun Syuk

AU - Kim, Pan Kyeom

AU - Kim, Mi Ryung

AU - Lee, Hyup Woo

AU - Kim, Chan Wha

PY - 2011/2

Y1 - 2011/2

N2 - Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.

AB - Objectives: To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. Design and methods: Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots in CVD patients were identified by ESI-Q-TOF MS/MS. Retinol binding protein 4 (RBP4), ceruloplasmin, and hemopexin were confirmed by Western blotting and RBP4 was further verified by ELISA. Results: Approximately, 400 spots were detected in each gel via comparisons of the serum proteome. Among these spots, 19 spots were selected and identified by ESI-Q-TOF MS/MS (P< 0.05). The expression levels of RBP4 and ceruloplasmin were higher in CVD patients by Western blotting. The level of immunoreactive RBP4 in CVD patients was higher than that in healthy subjects. Conclusions: The three proteins identified in the present study may constitute potential biomarkers for the diagnosis of CVD in patients.

KW - Biomarkers

KW - Cardiovascular disease

KW - Electrospray ionization quadrupole time-of-flight

KW - Proteome

KW - RBP4

KW - Two-dimensional gel electrophoresis

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Comparative analysis of serum proteomes of patients with cardiovascular disease

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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