Comparative analysis of the tear protein expression in blepharitis patients using two-dimensional electrophoresis

Bon S. Koo, Do Yeon Lee, Hyo Shin Ha, Jae Chan Kim, Chan Wha Kim

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Change in the expression of body fluid proteins is caused by many diseases or environmental disturbances. The changes in tear proteins are also associated with various pathological eye conditions. Especially, chronic blepharitis is one of the most common conditions seen in the ophthalmologist's office. However, there are no specific clinical diagnostic tests for blepharitis, and it is difficult to treat effectively. Therefore, the aim of this study was to screen prognostic or diagnostic marker tear proteins for blepharitis and investigate pathogenesis of this disease using proteomics techniques. The tear proteins expressed in patients suffering from blepharitis (patient, n = 19) and healthy volunteers (control, n = 27) were analyzed using the two-dimensional electrophoresis (2-DE) technique. The differentially expressed proteins in patients were identified with ESI-Q-TOF (electrospray-quadrupole-time-of-flight) mass spectrometry and confirmed with western blotting. Nine proteins in patient were down regulated about 50% compared to those of the control: serum albumin precursor, α-1 antitrypsin, lacritin precursor, lysozyme, Ig-κ chain VIII, prolactin inducible protein (PIP/GCDFP-15), cystatin-SA III, pyruvate kinase, and an unnamed protein. The use of the two-dimensional eletrophoretic technique could give more insight into the disease-related protein expression changes in tear fluids. Our findings reveal that the composition of tear proteins in blepharitis patients is different from that of healthy subjects and may provide further insights into the pathogenesis of blepharitis.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalJournal of Proteome Research
Volume4
Issue number3
DOIs
Publication statusPublished - 2005 May 1

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Blepharitis
Electrophoresis
Proteins
Healthy Volunteers
Salivary Cystatins
Pyruvate Kinase
Body fluids
Body Fluids
Muramidase
Tears
Routine Diagnostic Tests
Serum Albumin
Prolactin
Proteomics
Mass spectrometry
tear proteins
Mass Spectrometry
Western Blotting
Fluids
Chemical analysis

Keywords

  • Blepharitis
  • ESI-Q-TOF mass spectrometry
  • Tear protein
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics

Cite this

Comparative analysis of the tear protein expression in blepharitis patients using two-dimensional electrophoresis. / Koo, Bon S.; Lee, Do Yeon; Ha, Hyo Shin; Kim, Jae Chan; Kim, Chan Wha.

In: Journal of Proteome Research, Vol. 4, No. 3, 01.05.2005, p. 719-724.

Research output: Contribution to journalArticle

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