Comparative analysis of the transcriptome of injured nerve segments reveals spatiotemporal responses to neural damage in mice

Jung Eun Shin, Hongseok Ha, Eun Hye Cho, Yoon Ki Kim, Yongcheol Cho

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nerves are particularly vulnerable to damage due to their unique structure with meter-long axons. In the peripheral nervous system neurons and Schwann cells can activate the injury-response program that directs axons to either regenerate or degenerate after traumatic nerve injury. However, the differences between the genetic programs driving nerve regeneration and degeneration have not yet been described extensively. To understand these differences, in this study we have compared the injury-induced transcriptomic changes between the regenerating proximal segment and the degenerating distal segment of a transected nerve, at different post-injury time points. We analyzed the spatiotemporal dynamics of the mouse transcriptome using a sciatic nerve-injury model by means of RNA sequencing. The results of the differentially regulated genes (DEGs) analysis show that some DEG groups are similarly regulated in both proximal and distal segments, and primarily display a positive correlation. However, some DEG groups are exclusively regulated in either the proximal or the distal segment, suggesting that these DEG groups constitute a genetic network for distinguishing the regenerative and degenerative responses. In addition, our gene ontology analysis revealed an enrichment of particular biological processes in different phases and locations. Thus, our data provide a spatiotemporal profile of the transcriptomes that are differentially regulated in either regenerating or degenerating nerves, in vivo. The specific biological processes enriched in the DEG groups might delineate the injury-responsive program that induces contrasting regenerative and degenerative responses in different nerve segments.

Original languageEnglish
Pages (from-to)1195-1208
Number of pages14
JournalJournal of Comparative Neurology
Volume526
Issue number7
DOIs
Publication statusPublished - 2018 May 1

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Gene Expression Profiling
Wounds and Injuries
Biological Phenomena
Genes
Transcriptome
Axons
RNA Sequence Analysis
Nerve Degeneration
Nerve Regeneration
Gene Ontology
Schwann Cells
Peripheral Nervous System
Sciatic Nerve
Neurons

Keywords

  • axon degeneration
  • axon regeneration
  • RNA sequencing
  • RRID:IMSR_JAX:005304
  • RRID:SCR_001881
  • RRID:SCR_001915
  • RRID:SCR_011841
  • RRID:SCR_014568
  • RRID:SCR_014583
  • RRID:SCR_014597
  • RRID:SCR_015899
  • Schwann cell repair responses
  • sciatic nerve injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Comparative analysis of the transcriptome of injured nerve segments reveals spatiotemporal responses to neural damage in mice. / Shin, Jung Eun; Ha, Hongseok; Cho, Eun Hye; Kim, Yoon Ki; Cho, Yongcheol.

In: Journal of Comparative Neurology, Vol. 526, No. 7, 01.05.2018, p. 1195-1208.

Research output: Contribution to journalArticle

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