Comparative Biological Effects of Human Amnion and Chorion Membrane Extracts on Human Adipose-Derived Stromal Cells

Sik Namgoong, Hyunsu Lee, Jae Sun Lee, Seong-Ho Jeong, Seung-Kyu Han, Eun-Sang Dhong

Research output: Contribution to journalArticle

Abstract

Although therapies with human amnion/chorion are used to ameliorate acute and chronic wounds, it is unclear which component of the amnion/chorion tissue promotes wound healing. To characterize the comparative effects of amnion and chorion in wound healing, we used human adipose-derived stromal cells to assess cell viability, migration, and gel contraction after treatment with amnion membrane extract (AME) or chorion membrane extract (CME). We then correlated the possible effectors via AME and CME protein profiling, and compared them by enzyme-linked immunosorbent assay (ELISA), western blotting, and immunocytochemistry. Cell viability was significantly increased with 50 and 100 μg/mL AME treatment, but with CME treatment, a significant increase was only observed with 100 μg/mL. With CME treatment, cell migration was 2.22-fold greater than the control, and collagen gels showed 20% greater contraction. Compared to control, the expression levels of α-smooth muscle actin (SMA) and smooth muscle protein 22-alpha (SM22α) increased both with AME and CME treatments, whereas calponin expression decreased. Protein profiling revealed significantly higher tissue inhibitor of metalloproteinase-1 (TIMP-1), interleukin-8, exotoxin, and adiponectin levels in CME than in AME, and ELISA revealed 8-fold higher adiponectin levels in cells treated with CME than those treated with AME. Immunocytochemistry revealed that α-SMA, SM22α, and calponin were significantly higher in CME- than AME-treated cells; however, adiponectin treatment did not enhance α-SMA, SM22α, or calponin expression. In conclusion, amnion and chorion membrane extracts exerted differential effects on proliferation and contraction of human adipose-derived stromal cells. Amnion extract was superior at inducing cell proliferation and migration, whereas CME was superior at inducing cell contraction.

Original languageEnglish
Pages (from-to)947-954
Number of pages8
JournalThe Journal of craniofacial surgery
Volume30
Issue number3
DOIs
Publication statusPublished - 2019 May 1

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Chorion
Amnion
Stromal Cells
Membranes
Smooth Muscle
Muscle Proteins
Adiponectin
Cell Movement
Actins
Wound Healing
Therapeutics
Cell Survival
Gels
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Exotoxins
Tissue Inhibitor of Metalloproteinase-1

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Comparative Biological Effects of Human Amnion and Chorion Membrane Extracts on Human Adipose-Derived Stromal Cells. / Namgoong, Sik; Lee, Hyunsu; Lee, Jae Sun; Jeong, Seong-Ho; Han, Seung-Kyu; Dhong, Eun-Sang.

In: The Journal of craniofacial surgery, Vol. 30, No. 3, 01.05.2019, p. 947-954.

Research output: Contribution to journalArticle

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