Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation

Jaejoon Jung, Eugene L. Madsen, Che Ok Jeon, Woojun Park

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate 1,2-dioxygenase (nagI) and grows on gentisate, whereas other species cannot. Expression of the nagI gene was upregulated during gentisate utilization, and four downstream open reading frames (ORFs) were cotranscribed, supporting the notion that gentisate metabolism is a unique characteristic of strain DR1. The genomic analysis of strain DR1 provides additional insights into the function, ecology, and evolution of Acinetobacter species.

Original languageEnglish
Pages (from-to)7418-7424
Number of pages7
JournalApplied and Environmental Microbiology
Volume77
Issue number20
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Gentisates
Acinetobacter
biodegradation
genomics
gentisate 1,2-dioxygenase
Genome
genome
gene
DNA Transposable Elements
metabolism
Acinetobacter calcoaceticus
Acinetobacter baumannii
Biofilms
Microbial Drug Resistance
Protein Sorting Signals
Genomics
Metabolic Networks and Pathways
Ecology
Bacteriophages
Mental Competency

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation. / Jung, Jaejoon; Madsen, Eugene L.; Jeon, Che Ok; Park, Woojun.

In: Applied and Environmental Microbiology, Vol. 77, No. 20, 01.10.2011, p. 7418-7424.

Research output: Contribution to journalArticle

@article{7163f9b597714b7b95b13a3cfabddc86,
title = "Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation",
abstract = "The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate 1,2-dioxygenase (nagI) and grows on gentisate, whereas other species cannot. Expression of the nagI gene was upregulated during gentisate utilization, and four downstream open reading frames (ORFs) were cotranscribed, supporting the notion that gentisate metabolism is a unique characteristic of strain DR1. The genomic analysis of strain DR1 provides additional insights into the function, ecology, and evolution of Acinetobacter species.",
author = "Jaejoon Jung and Madsen, {Eugene L.} and Jeon, {Che Ok} and Woojun Park",
year = "2011",
month = "10",
day = "1",
doi = "10.1128/AEM.05231-11",
language = "English",
volume = "77",
pages = "7418--7424",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "20",

}

TY - JOUR

T1 - Comparative genomic analysis of Acinetobacter oleivorans DR1 To determine strain-specific genomic regions and gentisate biodegradation

AU - Jung, Jaejoon

AU - Madsen, Eugene L.

AU - Jeon, Che Ok

AU - Park, Woojun

PY - 2011/10/1

Y1 - 2011/10/1

N2 - The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate 1,2-dioxygenase (nagI) and grows on gentisate, whereas other species cannot. Expression of the nagI gene was upregulated during gentisate utilization, and four downstream open reading frames (ORFs) were cotranscribed, supporting the notion that gentisate metabolism is a unique characteristic of strain DR1. The genomic analysis of strain DR1 provides additional insights into the function, ecology, and evolution of Acinetobacter species.

AB - The comparative genomics of Acinetobacter oleivorans DR1 assayed with A. baylyi ADP1, A. calcoaceticus PHEA-2, and A. baumannii ATCC 17978 revealed that the incorporation of phage-related genomic regions and the absence of transposable elements have contributed to the large size (4.15 Mb) of the DR1 genome. A horizontally transferred genomic region and a higher proportion of transcriptional regulator- and signal peptide-coding genes were identified as characteristics of the DR1 genome. Incomplete glucose metabolism, metabolic pathways of aromatic compounds, biofilm formation, antibiotics and metal resistance, and natural competence genes were conserved in four compared genomes. Interestingly, only strain DR1 possesses gentisate 1,2-dioxygenase (nagI) and grows on gentisate, whereas other species cannot. Expression of the nagI gene was upregulated during gentisate utilization, and four downstream open reading frames (ORFs) were cotranscribed, supporting the notion that gentisate metabolism is a unique characteristic of strain DR1. The genomic analysis of strain DR1 provides additional insights into the function, ecology, and evolution of Acinetobacter species.

UR - http://www.scopus.com/inward/record.url?scp=82955239979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82955239979&partnerID=8YFLogxK

U2 - 10.1128/AEM.05231-11

DO - 10.1128/AEM.05231-11

M3 - Article

C2 - 21856821

AN - SCOPUS:82955239979

VL - 77

SP - 7418

EP - 7424

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 20

ER -