Comparative genomic analysis of Lactococcus garvieae phage WP-2, a new member of Picovirinae subfamily of Podoviridae

Seyed Mahdi Ghasemi, Majid Bouzari, Bo Hyun Yoon, Hyo-Ihl Chang

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

To date, a few numbers of bacteriophages that infect Lactococcus garvieae have been identified, but their complete genome sequences have not yet been investigated. For the first time, herein, the complete DNA sequence of a new phage of L. garvieae (phage WP-2) is reported and analyzed. The morphological characteristics indicated that the phage had a small isometric head along with a short and non-contractile tail, suggesting that WP-2 belongs to the family Podoviridae. Bioinformatic analysis revealed that phage WP-2 can be classified as a new member of Ahjdlikevirus in the Picovirinae subfamily because it had a small dsDNA of 18,899. bp with 24 open reading frames and a protein-primed DNA polymerase. The phage nucleotide sequence and predicted protein products have been identified to share very limited evidence of homology with complete genome and proteome of other phages. To our knowledge, this is the first Ahjdlikevirus bacteriophage which can infect a member of the Lactococcus genus.

Original languageEnglish
Pages (from-to)222-229
Number of pages8
JournalGene
Volume551
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

ASJC Scopus subject areas

  • Genetics

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