Comparative genomic hybridization array analysis and real time PCR reveals genomic alterations in squamous cell carcinomas of the lung

Yong Woo Choi, Jin Soo Choi, Long Tai Zheng, Yun Jeong Lim, Hyoung Kyu Yoon, Yeul Hong Kim, Young Pil Wang, Young Lim

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Genomic alterations have been identified in lung cancer tissues and reported in numerous studies. To analyze genomic aberrations in lung cancer patients, we used array comparative genomic hybridization (array CGH) in 14 squamous cell lung carcinoma (SqC) tissues. Copy number gain and loss in chromosomal regions were detected, and the corresponding genes were confirmed by real time PCR. Several frequently altered loci, including gain of 3q (36% of samples), were found. The most frequently identified losses were found at 14q32.33 (21% of samples). The relative degree of chromosomal change was analyzed using log2 ratios. High-level DNA amplifications (>0.8 log2 ratio) were detected at 20 regions in 1p, 2q, 3q, 4q, 6q, 7p, 8q, 9p, 10q, 12q, 14q and 19p. We found that the fold change levels were highest at EVI1 (3q26.2), LPP (3q27-28) and FHF-1 (3q28) gene loci. Our results show that array CGH is a useful tool for identification of gene alteration in lung cancer, and that the above-mentioned genes might represent potential candidate genes for pathogenesis and diagnosis of lung cancer.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalLung Cancer
Volume55
Issue number1
DOIs
Publication statusPublished - 2007 Jan

Keywords

  • Array CGH
  • Chromosomal aberration
  • Gene amplification
  • Lung cancer
  • Real time PCR
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

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