Abstract
Genomic alterations have been identified in lung cancer tissues and reported in numerous studies. To analyze genomic aberrations in lung cancer patients, we used array comparative genomic hybridization (array CGH) in 14 squamous cell lung carcinoma (SqC) tissues. Copy number gain and loss in chromosomal regions were detected, and the corresponding genes were confirmed by real time PCR. Several frequently altered loci, including gain of 3q (36% of samples), were found. The most frequently identified losses were found at 14q32.33 (21% of samples). The relative degree of chromosomal change was analyzed using log2 ratios. High-level DNA amplifications (>0.8 log2 ratio) were detected at 20 regions in 1p, 2q, 3q, 4q, 6q, 7p, 8q, 9p, 10q, 12q, 14q and 19p. We found that the fold change levels were highest at EVI1 (3q26.2), LPP (3q27-28) and FHF-1 (3q28) gene loci. Our results show that array CGH is a useful tool for identification of gene alteration in lung cancer, and that the above-mentioned genes might represent potential candidate genes for pathogenesis and diagnosis of lung cancer.
| Original language | English |
|---|---|
| Pages (from-to) | 43-51 |
| Number of pages | 9 |
| Journal | Lung Cancer |
| Volume | 55 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jan 1 |
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Keywords
- Array CGH
- Chromosomal aberration
- Gene amplification
- Lung cancer
- Real time PCR
- Squamous cell carcinoma
ASJC Scopus subject areas
- Oncology
Cite this
Comparative genomic hybridization array analysis and real time PCR reveals genomic alterations in squamous cell carcinomas of the lung. / Choi, Yong Woo; Choi, Jin Soo; Zheng, Long Tai; Lim, Yun Jeong; Yoon, Hyoung Kyu; Kim, Yeul Hong; Wang, Young Pil; Lim, Young.
In: Lung Cancer, Vol. 55, No. 1, 01.01.2007, p. 43-51.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative genomic hybridization array analysis and real time PCR reveals genomic alterations in squamous cell carcinomas of the lung
AU - Choi, Yong Woo
AU - Choi, Jin Soo
AU - Zheng, Long Tai
AU - Lim, Yun Jeong
AU - Yoon, Hyoung Kyu
AU - Kim, Yeul Hong
AU - Wang, Young Pil
AU - Lim, Young
PY - 2007/1/1
Y1 - 2007/1/1
N2 - Genomic alterations have been identified in lung cancer tissues and reported in numerous studies. To analyze genomic aberrations in lung cancer patients, we used array comparative genomic hybridization (array CGH) in 14 squamous cell lung carcinoma (SqC) tissues. Copy number gain and loss in chromosomal regions were detected, and the corresponding genes were confirmed by real time PCR. Several frequently altered loci, including gain of 3q (36% of samples), were found. The most frequently identified losses were found at 14q32.33 (21% of samples). The relative degree of chromosomal change was analyzed using log2 ratios. High-level DNA amplifications (>0.8 log2 ratio) were detected at 20 regions in 1p, 2q, 3q, 4q, 6q, 7p, 8q, 9p, 10q, 12q, 14q and 19p. We found that the fold change levels were highest at EVI1 (3q26.2), LPP (3q27-28) and FHF-1 (3q28) gene loci. Our results show that array CGH is a useful tool for identification of gene alteration in lung cancer, and that the above-mentioned genes might represent potential candidate genes for pathogenesis and diagnosis of lung cancer.
AB - Genomic alterations have been identified in lung cancer tissues and reported in numerous studies. To analyze genomic aberrations in lung cancer patients, we used array comparative genomic hybridization (array CGH) in 14 squamous cell lung carcinoma (SqC) tissues. Copy number gain and loss in chromosomal regions were detected, and the corresponding genes were confirmed by real time PCR. Several frequently altered loci, including gain of 3q (36% of samples), were found. The most frequently identified losses were found at 14q32.33 (21% of samples). The relative degree of chromosomal change was analyzed using log2 ratios. High-level DNA amplifications (>0.8 log2 ratio) were detected at 20 regions in 1p, 2q, 3q, 4q, 6q, 7p, 8q, 9p, 10q, 12q, 14q and 19p. We found that the fold change levels were highest at EVI1 (3q26.2), LPP (3q27-28) and FHF-1 (3q28) gene loci. Our results show that array CGH is a useful tool for identification of gene alteration in lung cancer, and that the above-mentioned genes might represent potential candidate genes for pathogenesis and diagnosis of lung cancer.
KW - Array CGH
KW - Chromosomal aberration
KW - Gene amplification
KW - Lung cancer
KW - Real time PCR
KW - Squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=33845961984&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845961984&partnerID=8YFLogxK
U2 - 10.1016/j.lungcan.2006.09.018
DO - 10.1016/j.lungcan.2006.09.018
M3 - Article
C2 - 17109992
AN - SCOPUS:33845961984
VL - 55
SP - 43
EP - 51
JO - Lung Cancer
JF - Lung Cancer
SN - 0169-5002
IS - 1
ER -