Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing deinococcus metallilatus strains

Chulwoo Park, Bora Shin, Wonjae Kim, Hoon Cheong, Soyoon Park, Woojun Park

Research output: Contribution to journalArticlepeer-review

Abstract

Deinococcus metallilatus MA1002 was exposed to ultraviolet radiation to generate mutants with enhanced biofilm production. Two strains (nos 5 and 6) were then selected based on their high biofilm formation, as well as their possession of higher con-centrations of extracellular matrix components (eDNA, protein and saccharides) than the wild-type (WT). Genomic sequencing revealed the presence of large genome deletions in a secondary chromosome in the mutants. Expression analyses of the WT and mutant strains indicated the upregulation of genes associated with exopolysaccharide synthesis and stress response. The mutant strains showed high mortality in glucose-supplemented (TYG) medium; however, cell death and biofilm formation were not increased in mutant cells grown under acetate-or glyoxylate-added media, suggesting that metabolic toxicity during glucose metabolism induced a high rate of cell death but improved biofilm formation in mutant strains. In damaged cells, eDNAs contributed to the enhanced biofilm formation of D. metallilatus.

Original languageEnglish
Article number000464
Pages (from-to)1-14
Number of pages14
JournalMicrobial genomics
Volume6
Issue number12
DOIs
Publication statusPublished - 2020

Keywords

  • Biofilm
  • CpG island
  • Deinococcus
  • Extracellular matrix
  • Genome deletion
  • Stress response

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology
  • Molecular Biology
  • Genetics

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