Comparative proteomic analysis of peripheral blood mononuclear cells from atopic dermatitis patients and healthy donors

Won Kon Kim, Hyun Ju Cho, Su In Ryu, Hyang Ran Hwang, Do Hyung Kim, Hye Young Ryu, Jin Woong Chung, Tae Yoon Kim, Byoung Chul Park, Kwang Hee Bae, Yong Ko, Sang Chul Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease that induces changes in various inflammatory skin cells. The prevalence of AD is as high as 18% in some regions of the world, and is steadily rising. However, the pathophysiology of AD is poorly understood. To identify the proteins involved in AD pathogenesis, a comparative proteomic analysis of protein expression in peripheral blood mononuclear cells isolated from AD patients and healthy donors was conducted. Significant changes were observed in the expressions of fourteen proteins, including the vinculin, PITPNB, and Filamin A proteins. Among the proteins, α-SNAP and FLNA decreased significantly, and PITPNB increased significantly in AD patients compared with control subjects; these findings were further confirmed by real-time PCR and Western blot analysis. The comparative proteome data may provide a valuable clue to further understand AD pathogenesis, and several differentially regulated proteins may be used as biomarkers for diagnosis and as target proteins for the development of novel drugs.

Original languageEnglish
Pages (from-to)597-603
Number of pages7
JournalJournal of Biochemistry and Molecular Biology
Volume41
Issue number8
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Atopic dermatitis
  • Biomarker
  • Peripheral blood mononuclear cells
  • Proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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