Comparative proteomic analysis of virulent Korean Mycobacterium tuberculosis K-strain with other mycobacteria strain following infection of U-937 macrophage

Weon Ryoo Sung, Kil Park Young, Sue Nie Park, Soo Shim Young, Hyunjeong Liew, Seongman Kang, Gill Han Bai

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.

Original languageEnglish
Pages (from-to)268-271
Number of pages4
JournalJournal of Microbiology
Volume45
Issue number3
Publication statusPublished - 2007 Jun

Keywords

  • 2-D PAGE
  • Human monocytic cell line U-937
  • MALDI-TOF-MS
  • MT2656
  • Mb1363
  • Mycobacterium tuberculosis K-strain
  • Proteomic analysis

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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