Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii

Yoon-E Choi, Hyemin Hwang, Hyun Soo Kim, Joon Woo Ahn, Won Joong Jeong, Ji Won Yang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalBioresource Technology
Volume145
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

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Keywords

  • Chlamydomonas reinhardtii
  • Comparative proteomics
  • Microalgal lipid biosynthesis
  • Random mutagenesis

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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