Comparative studies on metabolic rate and calpain/calpastatin activity between Hanwoo and Holstein beef

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Abstract

The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.

Original languageEnglish
Pages (from-to)1747-1753
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume15
Issue number12
Publication statusPublished - 2002 Dec 1

Fingerprint

calpastatin
calpain
Calpain
beef
Holstein
Temperature
Exsanguination
temperature
Muscles
muscles
Glycogen
Meat
longissimus muscle
meat quality
glycogen
Red Meat
bulls
breeds

Keywords

  • Calpain
  • Calpastatin
  • Hanwoo Beef
  • Holstein Beef
  • Metabolic Rate
  • Temperature Conditioning

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

@article{f675c91075b740f09273ceeb7b6ec9da,
title = "Comparative studies on metabolic rate and calpain/calpastatin activity between Hanwoo and Holstein beef",
abstract = "The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.",
keywords = "Calpain, Calpastatin, Hanwoo Beef, Holstein Beef, Metabolic Rate, Temperature Conditioning",
author = "Min-Suk Rhee and Ryu, {Y. C.} and Byoung-Chul Kim",
year = "2002",
month = "12",
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language = "English",
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pages = "1747--1753",
journal = "Asian-Australasian Journal of Animal Sciences",
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T1 - Comparative studies on metabolic rate and calpain/calpastatin activity between Hanwoo and Holstein beef

AU - Rhee, Min-Suk

AU - Ryu, Y. C.

AU - Kim, Byoung-Chul

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N2 - The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.

AB - The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.

KW - Calpain

KW - Calpastatin

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KW - Holstein Beef

KW - Metabolic Rate

KW - Temperature Conditioning

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