Abstract
The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.
| Original language | English |
|---|---|
| Pages (from-to) | 1747-1753 |
| Number of pages | 7 |
| Journal | Asian-Australasian Journal of Animal Sciences |
| Volume | 15 |
| Issue number | 12 |
| Publication status | Published - 2002 Dec 1 |
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Keywords
- Calpain
- Calpastatin
- Hanwoo Beef
- Holstein Beef
- Metabolic Rate
- Temperature Conditioning
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Comparative studies on metabolic rate and calpain/calpastatin activity between Hanwoo and Holstein beef. / Rhee, Min-Suk; Ryu, Y. C.; Kim, Byoung-Chul.
In: Asian-Australasian Journal of Animal Sciences, Vol. 15, No. 12, 01.12.2002, p. 1747-1753.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative studies on metabolic rate and calpain/calpastatin activity between Hanwoo and Holstein beef
AU - Rhee, Min-Suk
AU - Ryu, Y. C.
AU - Kim, Byoung-Chul
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.
AB - The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.
KW - Calpain
KW - Calpastatin
KW - Hanwoo Beef
KW - Holstein Beef
KW - Metabolic Rate
KW - Temperature Conditioning
UR - http://www.scopus.com/inward/record.url?scp=0036897546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036897546&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0036897546
VL - 15
SP - 1747
EP - 1753
JO - Asian-Australasian Journal of Animal Sciences
JF - Asian-Australasian Journal of Animal Sciences
SN - 1011-2367
IS - 12
ER -