TY - JOUR
T1 - Comparative studies on metabolic rate and calpain/calpastatin activity between Hanwoo and Holstein beef
AU - Rhee, M. S.
AU - Ryu, Y. C.
AU - Kim, B. C.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2002/12
Y1 - 2002/12
N2 - The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.
AB - The objectives of this study were to examine the effect of early short-term temperature conditioning on metabolic rate and calpain/calpastatin system and to compare variations in metabolic rate and calpain/calpastatin system between Hanwoo and Holstein beef. Longissimus thoracis et lumborum of the right carcass from 3 Hanwoo and 3 Holstein bulls were removed within 30 min of exsanguinations, cut into three pieces, and then temperature conditioned until 3 h postmortem (PM) at 2, 16, and 30°C, respectively. Rigor values (R-values; R248, R250, and R258), pH, muscle temperature, glycogen content, μ- and m-calpain activities, and calpastatin acitivity were measured at 1, 3, 9, and 24 h PM, respectively. Hanwoo beef had higher muscle temperature, faster metabolic rate at early PM stage in R-values, and lower μ-calpain activity than Holstein beef (p<0.05). The 30°C treatment maintained muscle temperature of 30°C until 3 h PM and resulted in faster pH decline at 3 and 9 h PM (p<0.05) than other treatments. The 16°C had higher (p<0.05) muscle temperature at 3 h PM than the 2°C, but no difference in all other traits was observed between the 2°C and the 16°C. Early shortterm temperature treatment used in this study was not sufficient to effectively activate calpain/calpastatin system. Correlations among all traits except m-calpain and muscle temperature were generally high (r>0.60; p<0.001). Among R-values, R258 had higher correlations with other metabolic traits than those of R248 and R250. These data suggest that early PM metabolic rate, μ-calpain activity, and calpastatin activity may be closely related to each other. Variations in metabolic rate and μ-calpain activity at early PM stage between Hanwoo and Holstein beef may imply variations in meat quality between both breeds.
KW - Calpain
KW - Calpastatin
KW - Hanwoo Beef
KW - Holstein Beef
KW - Metabolic Rate
KW - Temperature Conditioning
UR - http://www.scopus.com/inward/record.url?scp=0036897546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036897546&partnerID=8YFLogxK
U2 - 10.5713/ajas.2002.1747
DO - 10.5713/ajas.2002.1747
M3 - Article
AN - SCOPUS:0036897546
VL - 15
SP - 1747
EP - 1753
JO - Asian-Australasian Journal of Animal Sciences
JF - Asian-Australasian Journal of Animal Sciences
SN - 1011-2367
IS - 12
ER -