Comparative study of BMP-2 alone and combined with VEGF carried by hydrogel for maxillary alveolar bone regeneration

Sook Kyoung Kim, Tae Hyung Cho, Jeong Joon Han, In Sook Kim, Yongdoo Park, Soon Jung Hwang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.

Original languageEnglish
Pages (from-to)171-181
Number of pages11
JournalTissue Engineering and Regenerative Medicine
Volume13
Issue number2
DOIs
Publication statusPublished - 2016 Apr 1

Fingerprint

Bone Morphogenetic Protein 2
Bone Regeneration
Hydrogel
Maxilla
Hydrogels
Vascular Endothelial Growth Factor A
Bone
Proteins
Canidae
Porifera
Hyaluronic Acid
Osteogenesis
Intercellular Signaling Peptides and Proteins
Collagen
Staining and Labeling
Hyaluronic acid
Bone and Bones
Control Groups
von Willebrand Factor
Defects

Keywords

  • Bone morphogenetic protein-2
  • Bone regeneration
  • Maxillary alveolar bone defect
  • Synergistic effect
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Comparative study of BMP-2 alone and combined with VEGF carried by hydrogel for maxillary alveolar bone regeneration. / Kim, Sook Kyoung; Cho, Tae Hyung; Han, Jeong Joon; Kim, In Sook; Park, Yongdoo; Hwang, Soon Jung.

In: Tissue Engineering and Regenerative Medicine, Vol. 13, No. 2, 01.04.2016, p. 171-181.

Research output: Contribution to journalArticle

Kim, Sook Kyoung ; Cho, Tae Hyung ; Han, Jeong Joon ; Kim, In Sook ; Park, Yongdoo ; Hwang, Soon Jung. / Comparative study of BMP-2 alone and combined with VEGF carried by hydrogel for maxillary alveolar bone regeneration. In: Tissue Engineering and Regenerative Medicine. 2016 ; Vol. 13, No. 2. pp. 171-181.
@article{f66598fe984b49568bf1892246a1040b,
title = "Comparative study of BMP-2 alone and combined with VEGF carried by hydrogel for maxillary alveolar bone regeneration",
abstract = "The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.",
keywords = "Bone morphogenetic protein-2, Bone regeneration, Maxillary alveolar bone defect, Synergistic effect, Vascular endothelial growth factor",
author = "Kim, {Sook Kyoung} and Cho, {Tae Hyung} and Han, {Jeong Joon} and Kim, {In Sook} and Yongdoo Park and Hwang, {Soon Jung}",
year = "2016",
month = "4",
day = "1",
doi = "10.1007/s13770-015-0046-y",
language = "English",
volume = "13",
pages = "171--181",
journal = "Tissue Engineering and Regenerative Medicine",
issn = "1738-2696",
publisher = "Springer Science + Business Media",
number = "2",

}

TY - JOUR

T1 - Comparative study of BMP-2 alone and combined with VEGF carried by hydrogel for maxillary alveolar bone regeneration

AU - Kim, Sook Kyoung

AU - Cho, Tae Hyung

AU - Han, Jeong Joon

AU - Kim, In Sook

AU - Park, Yongdoo

AU - Hwang, Soon Jung

PY - 2016/4/1

Y1 - 2016/4/1

N2 - The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.

AB - The effect of vascular endothelial growth factor (VEGF) combined with bone morphogenetic protein-2 (BMP-2) for bone regeneration is still controversial as to whether or not VEGF has a synergistic or additive effect. This study attempted to evaluate the synergistic effect of VEGF and BMP-2 compared to BMP-2 alone for maxillary alveolar bone regeneration using collagen sponge/hydrogel complex sheets in a canine model. After mixing BMP-2 and VEGF with a hyaluronic acid-based hydrogel (HAH), the collagen sponge/hydrogel complex was transplanted into maxillary alveolar bone defects (n=14) after the extraction of canine upper first molars on both sides. Bone regeneration was evaluated in three groups (control group without growth factors, experimental groups I and II with BMP-2 alone and BMP-2 and VEGF, respectively) using micro-computed tomography and histological staining. The total amount of new bone formations and bone mineral density were significantly higher in the group with BMP-2 only and the group with BMP-2 combined with VEGF than it in the control group. The area with positive staining of von Willebrand factor bone defect was significantly greater in the group with BMP-2 only and with dual growth factors than the control. BMP-2 released from the HAH promoted new bone formation. However, the combination of BMP-2 and VEGF did not show a synergistic or additive effect on bone regeneration at canine maxillary alveolar bone defects.

KW - Bone morphogenetic protein-2

KW - Bone regeneration

KW - Maxillary alveolar bone defect

KW - Synergistic effect

KW - Vascular endothelial growth factor

UR - http://www.scopus.com/inward/record.url?scp=84962424471&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962424471&partnerID=8YFLogxK

U2 - 10.1007/s13770-015-0046-y

DO - 10.1007/s13770-015-0046-y

M3 - Article

AN - SCOPUS:84962424471

VL - 13

SP - 171

EP - 181

JO - Tissue Engineering and Regenerative Medicine

JF - Tissue Engineering and Regenerative Medicine

SN - 1738-2696

IS - 2

ER -