Comparative study of oxidative stress caused by anthracene and alkyl-anthracenes in caenorhabditis elegans

Ji Yeon Roh, Pil Gon Kim, Jung Hwan Kwon

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Oxidative stress was evaluated for anthracene (Ant) and alkyl-Ants (9-methylanthracene [9-MA] and 9,10-dimethylanthracene [9,10DMA]) in Caenorhabditis elegans to compare changes in toxicity due to the degree of alkylation. Worms were exposed at 1) the same external exposure concentration and 2) the maximum water-soluble concentration. Formation of reactive oxygen species, superoxide dismutase activity, total glutathione concentration, and lipid peroxidation were determined under constant exposure conditions using passive dosing. The expression of oxidative stress-related genes (daf-2, sir-2.1, daf-16, sod-1, sod-2, sod-3 and cytochrome 35A/C family genes) was also investigated to identify and compare changes in the genetic responses of C. elegans exposed to Ant and alkyl-Ant. At the same external concentration, 9,10-DMA induced the greatest oxidative stress, as evidenced by all indicators, except for lipid peroxidation, followed by 9-MA and Ant. Interestingly, 9,10-DMA led to greater oxidative stress than 9-MA and Ant when worms were exposed to the maximum water-soluble concentration, although the maximum water-soluble concentration of 9,10DMA is the lowest. Increased oxidative stress by alkyl-Ants would be attributed to higher lipid-water partition coefficient and the π electron density in aromatic rings by alkyl substitution, although this supposition requires further confirmation.

Original languageEnglish
Article numbere2018006
Pages (from-to)1-8
Number of pages8
JournalEnvironmental Health and Toxicology
Volume33
Issue number1
DOIs
Publication statusPublished - 2018

Keywords

  • Caenorhabditis elegans
  • Gene expression
  • Oxidative stress
  • Passive dosing
  • Polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Toxicology
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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