Comparative study on high fat diet-induced 4-hydroxy-2E-nonenal adducts in the hippocampal CA1 region of C57BL/6N and C3H/HeN mice

In Koo Hwang, Il Yong Kim, Yo Na Kim, Sun Shin Yi, In Sun Park, Bon Hong Min, Ho Kyung Doo, Se Young Ahn, Yong Suk Kim, In Se Lee, Yeo Sung Yoon, Je Kyung Seong

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Abstract

In the present study, we investigated the influences of a high fat diet (HD) fed for 12 weeks, on lipid peroxidation and antioxidant enzyme using 4-hydroxy-2E-nonenal (HNE)-modified proteins (HNE-mp) and Cu,Zn-superoxide dismutase (SOD1) in the hippocampal CA1 region (CA1) in C57BL/6N and C3H/HeN mice. Body weights and body weight gains were significantly higher in HD fed C57BL/6N mice than in low fat diet (LD) fed C57BL/6N and LD or HD fed C3H/HeN mice. In the HD fed C57BL/6N and C3H/HeN mice, HNE-mp immunoreactivity and protein levels were much higher than in the LD fed C57BL/6N or C3H/HeN mice. In particular, HNE-mp immunoreactivity and protein levels in HD fed C57BL/6N mice was higher than that in the HD fed C3H/HeN mice. SOD1 immunoreaction was detected in the non-pyramidal cells of C57BL/6N mice, while in the C3H/HeN mice SOD1 immunoreaction was observed in CA1 pyramidal cells. The SOD1 immunoreactivity in the LD fed C57BL/6N and C3H/HeN mice was slightly, but not significantly decreased compared to that in the HD fed C57BL/6N and C3H/HeN mice, respectively. In addition, ionized calcium-binding adapter molecule 1 (Iba-1) immunoreactive microglia in the HD fed C57BL/6N showed hypertrophy of cytoplasm, which is the characteristics of activated microglia. These results suggest that HD fed C57BL/6N mice are more susceptible to lipid peroxidation in the CA1 than in LD fed C57BL/6N and LD or HD fed C3H/HeN mice without any differences of SOD1 expression.

Original languageEnglish
Pages (from-to)964-972
Number of pages9
JournalNeurochemical Research
Volume34
Issue number5
DOIs
Publication statusPublished - 2009 May

Keywords

  • 4-Hydroxynonenal
  • Body weight
  • C3H/HeN
  • C57BL/6N
  • High fat diet
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Hwang, I. K., Kim, I. Y., Kim, Y. N., Yi, S. S., Park, I. S., Min, B. H., Doo, H. K., Ahn, S. Y., Kim, Y. S., Lee, I. S., Yoon, Y. S., & Seong, J. K. (2009). Comparative study on high fat diet-induced 4-hydroxy-2E-nonenal adducts in the hippocampal CA1 region of C57BL/6N and C3H/HeN mice. Neurochemical Research, 34(5), 964-972. https://doi.org/10.1007/s11064-008-9846-y