Comparison between size exclusion chromatography and micro-batch analyses of corn starches in DMSO using light scattering detector

Jung Ah Han, Hyesook Lim, Seung Taik Lim

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The molecular structure of corn starches different in amylose content (waxy, normal, and high-amylose) was analyzed in 90% dimethyl sulfoxide (DMSO) solution by refractive index (RI) and multi-angle laser light scattering (MALLS) detectors. The starch sample solutions were measured either by medium-pressure size exclusion chromatography (MPSEC) or by the micro-batch mode. For waxy corn starch, the average molar mass (Mw) and radius of gyration (R g) values were similar in both methods. However, for normal and high-amylose corn starches, Mw measured by the micro-batch mode was 2-4 times greater than that by the chromatographic method, although R g values obtained from both methods were not very different. The Mw difference was the greater the higher the amylose content of starch.

Original languageEnglish
Pages (from-to)262-267
Number of pages6
JournalStarch/Staerke
Volume57
Issue number6
DOIs
Publication statusPublished - 2005 Jun 1

Fingerprint

Size exclusion chromatography
light scattering
dimethyl sulfoxide
Dimethyl Sulfoxide
corn starch
Amylose
amylose
Starch
Light scattering
detectors
Zea mays
Gel Chromatography
Detectors
Light
starch
waxy corn
refractive index
Refractometry
chemical structure
Molar mass

Keywords

  • Average molar mass
  • Corn starch
  • DMSO
  • Radius of gyration

ASJC Scopus subject areas

  • Food Science

Cite this

Comparison between size exclusion chromatography and micro-batch analyses of corn starches in DMSO using light scattering detector. / Han, Jung Ah; Lim, Hyesook; Lim, Seung Taik.

In: Starch/Staerke, Vol. 57, No. 6, 01.06.2005, p. 262-267.

Research output: Contribution to journalArticle

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