Comparison between size exclusion chromatography and micro-batch analyses of corn starches in DMSO using light scattering detector

Jung Ah Han, Hyesook Lim, Seung Taik Lim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The molecular structure of corn starches different in amylose content (waxy, normal, and high-amylose) was analyzed in 90% dimethyl sulfoxide (DMSO) solution by refractive index (RI) and multi-angle laser light scattering (MALLS) detectors. The starch sample solutions were measured either by medium-pressure size exclusion chromatography (MPSEC) or by the micro-batch mode. For waxy corn starch, the average molar mass (Mw) and radius of gyration (R g) values were similar in both methods. However, for normal and high-amylose corn starches, Mw measured by the micro-batch mode was 2-4 times greater than that by the chromatographic method, although R g values obtained from both methods were not very different. The Mw difference was the greater the higher the amylose content of starch.

Original languageEnglish
Pages (from-to)262-267
Number of pages6
JournalStarch/Staerke
Volume57
Issue number6
DOIs
Publication statusPublished - 2005 Jun

Keywords

  • Average molar mass
  • Corn starch
  • DMSO
  • Radius of gyration

ASJC Scopus subject areas

  • Food Science
  • Organic Chemistry

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