Abstract
The molecular structure of corn starches different in amylose content (waxy, normal, and high-amylose) was analyzed in 90% dimethyl sulfoxide (DMSO) solution by refractive index (RI) and multi-angle laser light scattering (MALLS) detectors. The starch sample solutions were measured either by medium-pressure size exclusion chromatography (MPSEC) or by the micro-batch mode. For waxy corn starch, the average molar mass (Mw) and radius of gyration (R g) values were similar in both methods. However, for normal and high-amylose corn starches, Mw measured by the micro-batch mode was 2-4 times greater than that by the chromatographic method, although R g values obtained from both methods were not very different. The Mw difference was the greater the higher the amylose content of starch.
| Original language | English |
|---|---|
| Pages (from-to) | 262-267 |
| Number of pages | 6 |
| Journal | Starch/Staerke |
| Volume | 57 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2005 Jun 1 |
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Keywords
- Average molar mass
- Corn starch
- DMSO
- Radius of gyration
ASJC Scopus subject areas
- Food Science
Cite this
Comparison between size exclusion chromatography and micro-batch analyses of corn starches in DMSO using light scattering detector. / Han, Jung Ah; Lim, Hyesook; Lim, Seung Taik.
In: Starch/Staerke, Vol. 57, No. 6, 01.06.2005, p. 262-267.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison between size exclusion chromatography and micro-batch analyses of corn starches in DMSO using light scattering detector
AU - Han, Jung Ah
AU - Lim, Hyesook
AU - Lim, Seung Taik
PY - 2005/6/1
Y1 - 2005/6/1
N2 - The molecular structure of corn starches different in amylose content (waxy, normal, and high-amylose) was analyzed in 90% dimethyl sulfoxide (DMSO) solution by refractive index (RI) and multi-angle laser light scattering (MALLS) detectors. The starch sample solutions were measured either by medium-pressure size exclusion chromatography (MPSEC) or by the micro-batch mode. For waxy corn starch, the average molar mass (Mw) and radius of gyration (R g) values were similar in both methods. However, for normal and high-amylose corn starches, Mw measured by the micro-batch mode was 2-4 times greater than that by the chromatographic method, although R g values obtained from both methods were not very different. The Mw difference was the greater the higher the amylose content of starch.
AB - The molecular structure of corn starches different in amylose content (waxy, normal, and high-amylose) was analyzed in 90% dimethyl sulfoxide (DMSO) solution by refractive index (RI) and multi-angle laser light scattering (MALLS) detectors. The starch sample solutions were measured either by medium-pressure size exclusion chromatography (MPSEC) or by the micro-batch mode. For waxy corn starch, the average molar mass (Mw) and radius of gyration (R g) values were similar in both methods. However, for normal and high-amylose corn starches, Mw measured by the micro-batch mode was 2-4 times greater than that by the chromatographic method, although R g values obtained from both methods were not very different. The Mw difference was the greater the higher the amylose content of starch.
KW - Average molar mass
KW - Corn starch
KW - DMSO
KW - Radius of gyration
UR - http://www.scopus.com/inward/record.url?scp=21244450741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21244450741&partnerID=8YFLogxK
U2 - 10.1002/star.200400365
DO - 10.1002/star.200400365
M3 - Article
AN - SCOPUS:21244450741
VL - 57
SP - 262
EP - 267
JO - Starch/Staerke
JF - Starch/Staerke
SN - 0038-9056
IS - 6
ER -