Abstract
Mass spectrometry (MS) is a very powerful instrument that can be used to analyze a wide range of materials such as proteins, peptides, DNA, drugs, and polymers. The process typically involves either chemical or electron (impact) ionization of the analyte. The resulting charged species or fragment is subsequently identified by the detector. Usually, single mass uses source-induced dissociation (SID), whereas mass/mass uses collision-induced dissociation (CID) to analyze the chemical fragmentations Each technique has its own advantages and disadvantages. While CID is most effective for the analysis of pure substances, multiple- step MS is a powerful technique to get structural data. Analysis of veterinary drugs ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline serves to highlight the slight differences between SID and CID. For example, minor differences were observed between ciprofloxacin and oxytetracycline via SID or CID. However, distinct fragmentation patterns were observed for ampicllin depending on the analysis method. Both SID and CID showed similar fragmentation spectra but different signal intensities for chloramphenicol. There are several factors that can influence the fragmentation spectra, such as the collision energy, major precursor ion, electrospray mode (positive or negative), and sample homogeneity. Therefore, one must select a fragmentation method on an empirical and case-by-case basis.
| Original language | English |
|---|---|
| Pages (from-to) | 107-114 |
| Number of pages | 8 |
| Journal | Toxicological Research |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2013 Oct 29 |
Fingerprint
Keywords
- Ampicillin
- Chloramphenicol
- Ciprofloxacin
- Mass spectrometry
- Oxytetracycline
ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Comparison between source-induced dissociation and collision-induced dissociation of ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline via mass spectrometry. / Lee, Seung Ha; Choi, DalWoong.
In: Toxicological Research, Vol. 29, No. 2, 29.10.2013, p. 107-114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison between source-induced dissociation and collision-induced dissociation of ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline via mass spectrometry
AU - Lee, Seung Ha
AU - Choi, DalWoong
PY - 2013/10/29
Y1 - 2013/10/29
N2 - Mass spectrometry (MS) is a very powerful instrument that can be used to analyze a wide range of materials such as proteins, peptides, DNA, drugs, and polymers. The process typically involves either chemical or electron (impact) ionization of the analyte. The resulting charged species or fragment is subsequently identified by the detector. Usually, single mass uses source-induced dissociation (SID), whereas mass/mass uses collision-induced dissociation (CID) to analyze the chemical fragmentations Each technique has its own advantages and disadvantages. While CID is most effective for the analysis of pure substances, multiple- step MS is a powerful technique to get structural data. Analysis of veterinary drugs ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline serves to highlight the slight differences between SID and CID. For example, minor differences were observed between ciprofloxacin and oxytetracycline via SID or CID. However, distinct fragmentation patterns were observed for ampicllin depending on the analysis method. Both SID and CID showed similar fragmentation spectra but different signal intensities for chloramphenicol. There are several factors that can influence the fragmentation spectra, such as the collision energy, major precursor ion, electrospray mode (positive or negative), and sample homogeneity. Therefore, one must select a fragmentation method on an empirical and case-by-case basis.
AB - Mass spectrometry (MS) is a very powerful instrument that can be used to analyze a wide range of materials such as proteins, peptides, DNA, drugs, and polymers. The process typically involves either chemical or electron (impact) ionization of the analyte. The resulting charged species or fragment is subsequently identified by the detector. Usually, single mass uses source-induced dissociation (SID), whereas mass/mass uses collision-induced dissociation (CID) to analyze the chemical fragmentations Each technique has its own advantages and disadvantages. While CID is most effective for the analysis of pure substances, multiple- step MS is a powerful technique to get structural data. Analysis of veterinary drugs ampicillin, chloramphenicol, ciprofloxacin, and oxytetracycline serves to highlight the slight differences between SID and CID. For example, minor differences were observed between ciprofloxacin and oxytetracycline via SID or CID. However, distinct fragmentation patterns were observed for ampicllin depending on the analysis method. Both SID and CID showed similar fragmentation spectra but different signal intensities for chloramphenicol. There are several factors that can influence the fragmentation spectra, such as the collision energy, major precursor ion, electrospray mode (positive or negative), and sample homogeneity. Therefore, one must select a fragmentation method on an empirical and case-by-case basis.
KW - Ampicillin
KW - Chloramphenicol
KW - Ciprofloxacin
KW - Mass spectrometry
KW - Oxytetracycline
UR - http://www.scopus.com/inward/record.url?scp=84886292092&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886292092&partnerID=8YFLogxK
U2 - 10.5487/TR.2013.29.2.107
DO - 10.5487/TR.2013.29.2.107
M3 - Article
AN - SCOPUS:84886292092
VL - 29
SP - 107
EP - 114
JO - Toxicological Research
JF - Toxicological Research
SN - 1976-8257
IS - 2
ER -