Comparison between the SFTS-QS Kit and the PowerChek SFTSV real-time PCR kit for the detection of severe fever with thrombocytopenia syndrome virus

In Young Yoo, Ji Youn Kim, Young Kyung Yoon, Hee Jae Huh, Nam Yong Lee

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now renamed Huaiyangshan banyangvirus). SFTS-QS is a qualitative real-time reverse transcription PCR assay based on lab-on-a- chip technology. We evaluated the performance of the SFTS-QS kit and compared it with that of the PowerChek SFTSV Real-time PCR kit (PowerChek; Kogene Biotech, Seoul, Korea). A total of 117 serum samples were simultaneously assayed using the SFTS-QS and PowerChek kits. Sanger sequencing targeting the S and M segments of SFTSV was performed as the reference method. The total turnaround time of the two kits was compared. The SFTS-QS results agreed with those of PowerChek with a kappa value of 0.92. The diagnostic sensitivity and specificity of the SFTS-QS kit were both 100% (14/14 and 103/103, respectively), whereas those of the PowerChek kit were 100% (14/14) and 98.1% (101/103), respectively. The results of SFTS-QS and PowerChek were comparable; however, the SFTS-QS kit required a shorter total turnaround time. The SFTS-QS kit produced accurate and fast results and thus could serve as a useful tool for detecting SFTSV.

    Original languageEnglish
    Pages (from-to)317-320
    Number of pages4
    JournalAnnals of laboratory medicine
    Volume40
    Issue number4
    DOIs
    Publication statusPublished - 2020

    Keywords

    • Huaiyangshan banyangvirus
    • Performance
    • PowerChek SFTSV Real-time PCR kit
    • SFTS virus
    • SFTS-QS kit
    • Turnaround time

    ASJC Scopus subject areas

    • Clinical Biochemistry
    • Biochemistry, medical

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