Comparison between the SFTS-QS Kit and the PowerChek SFTSV real-time PCR kit for the detection of severe fever with thrombocytopenia syndrome virus

In Young Yoo, Ji Youn Kim, Young Kyung Yoon, Hee Jae Huh, Nam Yong Lee

Research output: Contribution to journalArticle

Abstract

The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now renamed Huaiyangshan banyangvirus). SFTS-QS is a qualitative real-time reverse transcription PCR assay based on lab-on-a- chip technology. We evaluated the performance of the SFTS-QS kit and compared it with that of the PowerChek SFTSV Real-time PCR kit (PowerChek; Kogene Biotech, Seoul, Korea). A total of 117 serum samples were simultaneously assayed using the SFTS-QS and PowerChek kits. Sanger sequencing targeting the S and M segments of SFTSV was performed as the reference method. The total turnaround time of the two kits was compared. The SFTS-QS results agreed with those of PowerChek with a kappa value of 0.92. The diagnostic sensitivity and specificity of the SFTS-QS kit were both 100% (14/14 and 103/103, respectively), whereas those of the PowerChek kit were 100% (14/14) and 98.1% (101/103), respectively. The results of SFTS-QS and PowerChek were comparable; however, the SFTS-QS kit required a shorter total turnaround time. The SFTS-QS kit produced accurate and fast results and thus could serve as a useful tool for detecting SFTSV.

Original languageEnglish
Pages (from-to)317-320
Number of pages4
JournalAnnals of laboratory medicine
Volume40
Issue number4
DOIs
Publication statusPublished - 2020

Keywords

  • Huaiyangshan banyangvirus
  • Performance
  • PowerChek SFTSV Real-time PCR kit
  • SFTS virus
  • SFTS-QS kit
  • Turnaround time

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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