Comparison of bradykinin- and platelet-derived growth factor-induced phosphoinositide turnover in NIH 3T3 cells

Kee H. Lee, Yong W. Ryu, Young Do Yoo, Dong Hoon Bai, Ju Hyun Yu, Chang M. Kim

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Abstract

Phosphoinositide turnover in response to platelet-derived growth factor, epidermal growth factor, and bradykinin was evaluated in NIH 3T3 cells. Platelet-derived growth factor and bradykinin induced a significant increase in incorporation of 32P into phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP), and phosphatidylinositol 4,5-bisphosphate (PIP2) in serum-starved NIH 3T3 cells. However, epidermal growth factor increased incorporation of 32P into these phosphoinositides by only a small amount. Stimulation with platelet-derived growth factor, not bradykinin, caused a rapid elevation of PI and PIP kinase activities that were maximally activated within 10 min. The maximal levels of their elevation in cells with platelet-derived growth factor stimulation were 3.2-fold for PI kinase, and 2.1-fold for PIP kinase. Short term pretreatment of NIH 3T3 cells with phorbol 12-myristate 13-acetate, activator of protein kinase C, caused an approximately 60% decrease in platelet-derived growth factor-induced PI kinase activities, indicating the feedback regulation of phosphoinositide turnover by protein kinase C. These results suggest that although the enhancement of phosphoinositide turnover is a rapidly occurring response in platelet-derived growth factor- or bradykinin-stimulated NIH 3T3 cells, phosphoinositide kinases may be associated with initial signal transduction pathway relevant to platelet-derived growth factor but not to bradykinin.

Original languageEnglish
Pages (from-to)549-554
Number of pages6
JournalJournal of Biochemistry and Molecular Biology
Volume29
Issue number6
Publication statusPublished - 1996 Nov 30
Externally publishedYes

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Keywords

  • Bradykinin
  • Phosphatidylinositol 4-monophosphate kinase
  • Phosphatidylinositol kinase
  • Phosphoinositide turnover
  • Platelet-derived growth factor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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