Comparison of conjunctival staining between lissamine green and yellow filtered fluorescein sodium

Youngsub Eom, Jong Suk Lee, Hyung Keun Lee, Hyo Myung Kim, Jong-Suk Song

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Abstract Objective To compare conjunctival staining between lissamine green and fluorescein sodium using a yellow barrier filter. Design Retrospective cross-sectional study. Participants Thirteen eyes of 13 patients with dry eyes. Methods All patients underwent ocular surface staining with fluorescein sodium and lissamine green. Ocular surface staining with fluorescein sodium was observed through slit-lamp examination under cobalt blue illumination and a yellow filter. Temporal (0-5) and nasal (0-5) conjunctival staining grade was separately graded according to the Oxford scheme. The contrast score was defined as the absolute difference between mean grey values of punctate staining and background. Conjunctival staining grade and contrast scores were compared between these 2 staining methods. Results The sum of the conjunctival staining grade (0-10) for the nasal and temporal conjunctiva was 4.7 ± 2.5 for fluorescein sodium, which was significantly higher than that of lissamine green, 4.1 ± 2.5 (p = 0.005). The contrast score of temporal and nasal conjunctival staining was 34.6 ± 12.0 and 34.7 ± 10.3, respectively, for fluorescein sodium, which was also significantly greater than for lissamine green (23.4 ± 8.1 and 21.0 ± 9.0, respectively; p = 0.003 and p = 0.003, respectively). Conclusions Conjunctival staining with fluorescein sodium and a yellow filter seems to be more sensitive for the detection of conjunctival damage than lissamine green. Fluorescein staining with the yellow filter has the advantage of simultaneous observation of both corneal and conjunctival damage in patients with dry eye without the need for additional vital staining.

Original languageEnglish
Article number761
Pages (from-to)273-277
Number of pages5
JournalCanadian Journal of Ophthalmology
Volume50
Issue number4
DOIs
Publication statusPublished - 2015 Jan 1

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Fluorescein
Staining and Labeling
Nose
Conjunctiva
Cobalt
Lighting
Cross-Sectional Studies
Observation

ASJC Scopus subject areas

  • Medicine(all)
  • Ophthalmology

Cite this

Comparison of conjunctival staining between lissamine green and yellow filtered fluorescein sodium. / Eom, Youngsub; Lee, Jong Suk; Keun Lee, Hyung; Kim, Hyo Myung; Song, Jong-Suk.

In: Canadian Journal of Ophthalmology, Vol. 50, No. 4, 761, 01.01.2015, p. 273-277.

Research output: Contribution to journalArticle

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abstract = "Abstract Objective To compare conjunctival staining between lissamine green and fluorescein sodium using a yellow barrier filter. Design Retrospective cross-sectional study. Participants Thirteen eyes of 13 patients with dry eyes. Methods All patients underwent ocular surface staining with fluorescein sodium and lissamine green. Ocular surface staining with fluorescein sodium was observed through slit-lamp examination under cobalt blue illumination and a yellow filter. Temporal (0-5) and nasal (0-5) conjunctival staining grade was separately graded according to the Oxford scheme. The contrast score was defined as the absolute difference between mean grey values of punctate staining and background. Conjunctival staining grade and contrast scores were compared between these 2 staining methods. Results The sum of the conjunctival staining grade (0-10) for the nasal and temporal conjunctiva was 4.7 ± 2.5 for fluorescein sodium, which was significantly higher than that of lissamine green, 4.1 ± 2.5 (p = 0.005). The contrast score of temporal and nasal conjunctival staining was 34.6 ± 12.0 and 34.7 ± 10.3, respectively, for fluorescein sodium, which was also significantly greater than for lissamine green (23.4 ± 8.1 and 21.0 ± 9.0, respectively; p = 0.003 and p = 0.003, respectively). Conclusions Conjunctival staining with fluorescein sodium and a yellow filter seems to be more sensitive for the detection of conjunctival damage than lissamine green. Fluorescein staining with the yellow filter has the advantage of simultaneous observation of both corneal and conjunctival damage in patients with dry eye without the need for additional vital staining.",
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N2 - Abstract Objective To compare conjunctival staining between lissamine green and fluorescein sodium using a yellow barrier filter. Design Retrospective cross-sectional study. Participants Thirteen eyes of 13 patients with dry eyes. Methods All patients underwent ocular surface staining with fluorescein sodium and lissamine green. Ocular surface staining with fluorescein sodium was observed through slit-lamp examination under cobalt blue illumination and a yellow filter. Temporal (0-5) and nasal (0-5) conjunctival staining grade was separately graded according to the Oxford scheme. The contrast score was defined as the absolute difference between mean grey values of punctate staining and background. Conjunctival staining grade and contrast scores were compared between these 2 staining methods. Results The sum of the conjunctival staining grade (0-10) for the nasal and temporal conjunctiva was 4.7 ± 2.5 for fluorescein sodium, which was significantly higher than that of lissamine green, 4.1 ± 2.5 (p = 0.005). The contrast score of temporal and nasal conjunctival staining was 34.6 ± 12.0 and 34.7 ± 10.3, respectively, for fluorescein sodium, which was also significantly greater than for lissamine green (23.4 ± 8.1 and 21.0 ± 9.0, respectively; p = 0.003 and p = 0.003, respectively). Conclusions Conjunctival staining with fluorescein sodium and a yellow filter seems to be more sensitive for the detection of conjunctival damage than lissamine green. Fluorescein staining with the yellow filter has the advantage of simultaneous observation of both corneal and conjunctival damage in patients with dry eye without the need for additional vital staining.

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