Comparison of light-transmission and -backscattering methods in the measurement of red blood cell aggregation

Jeong Hun Nam, Yijie Yang, Seok Chung, Sehyun Shin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Light-transmission and light-backscattering methods are commonly used to determine red-blood-cell (RBC) aggregation. Even though the results reveal good correlations between the parameters that are measured by these two methods, the methods themselves yield quite different values. The objective of this research is to investigate and delineate the characteristics of the two optical methods. We measured RBC aggregation by using a newly developed microchipbased aggregometer. An orthogonal polarization technique, wherein multiple scattering causes polarized light to be depolarized and passed through an orthogonal polarizer, was applied to the backscattering method. Our results were also compared to those of conventional aggregometers [laser-assisted optical rotational cell analyzer (LORCA)], and revealed that the backscattering method yielded higher aggregation indices than the transmission method and LORCA. However, the backscattering method with orthogonal polarization yielded the same values of aggregation indices as the transmission method. These agreements between the two methods were also found in measurements of RBC aggregability in various concentrations of dextran solutions.

Original languageEnglish
Article number027003
JournalJournal of Biomedical Optics
Volume15
Issue number2
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Cell Aggregation
light transmission
erythrocytes
Backscattering
Light transmission
backscattering
Blood
Agglomeration
Erythrocytes
Cells
Light
analyzers
Polarization
dextrans
Dextran
Lasers
Multiple scattering
polarization
Light polarization
Dextrans

Keywords

  • Aggregation
  • Backscattering
  • Erythrocyte
  • Orthogonal polarization
  • Transmission

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Comparison of light-transmission and -backscattering methods in the measurement of red blood cell aggregation. / Nam, Jeong Hun; Yang, Yijie; Chung, Seok; Shin, Sehyun.

In: Journal of Biomedical Optics, Vol. 15, No. 2, 027003, 01.12.2010.

Research output: Contribution to journalArticle

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