Comparison of mesenchymal-like stem/progenitor cells derived from supernumerary teeth with stem cells from human exfoliated deciduous teeth

Sunray Lee, Soyoun An, Tae Hoon Kang, Kyung Hye Kim, Nicole Hyesoo Chang, Seongman Kang, Chang Kon Kwak, Hyun Sook Park

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Aims: Dental tissue has been the focus of attention as an easily accessible postnatal tissue source of high-quality stem cells. Since the first report on the dental pulp stem cells (DPSCs) from permanent third molar teeth, stem cells from human exfoliated deciduous teeth (SHED) were identified as a population distinct from DPSCs. In this study, we compared DPSCs from supernumerary teeth and SHED in three age- and sex-matched patients. Patients & methods: Dental samples were obtained from the three patients, who were 6 years old and male, with the parental consent of the three donors, and then isolated cells from dental pulp for comparative analysis between supernumerary DPSCs and SHED. Results: Colony-forming unit fibroblast levels and the proliferation rate of supernumerary DPSCs were slightly lower than that of SHED. The expression of cell surface antigens in supernumerary DPSCs and SHED were almost identical. Cells were mainly expressing endogenous mesodermal and ectodermal lineage markers. Differentiation capacity to osteogenic, adipogenic and chondrogenic lineage was similar in the SHED and supernumerary DPSCs. Migration assay revealed that both supernumerary DPSCs and SHED rapidly migrated toward wounded areas. Supernumerary DPSCs were altered in cell growth after storage for 2 years. Specially, the population doubling time of supernumerary DPSCs increased while that of SHED remained nearly unchanged. Conclusion: Both supernumerary teeth and deciduous teeth share many characteristics, such as highly proliferative clonogenic cells with a similar immunophenotype to that of mesenchymal stem cells, although they are inferior to SHED for long-term banking. Our findings suggest that supernumerary teeth are also easily accessible and noninvasive sources of postnatal stem cells with multipotency and regenerative capacity.

Original languageEnglish
Pages (from-to)689-699
Number of pages11
JournalRegenerative Medicine
Volume6
Issue number6
DOIs
Publication statusPublished - 2011 Nov

Keywords

  • dental pulp cell
  • mesenchymal stem cell
  • stem cells from human exfoliated deciduous teeth
  • supernumerary dental pulp stem cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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