Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources

Dae Kyun Im, Seok Hun Yun, Joon Young Jung, Jinwon Lee, Min-Kyu Oh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Metabolite profiling was conducted to monitor the metabolic differences of the Ralstonia eutropha H16, polyhydroxybutyrate (PHB) producing strain, when grown on different sole carbon sources and to identify the effect of the deletion of the PHB production pathway. The combination of rapid filtration and boiling ethanol extraction successfully extracted intracellular metabolites. Accumulation of acetyl-CoA was detected when the strain was grown on fructose. Whereas, lower adenylate energy charge (AEC) ratio and cell mass was identified on the cells grown on acetate. No significant difference was detected between the wild type and mutant strain except metabolites in the PHB synthetic pathway. Increased PHB production is expected on fructose due to the accumulation of AcCoA, while it is more difficult to improve the production on acetate because of lower metabolic precursor level and AEC ratio.

Original languageEnglish
Pages (from-to)797-805
Number of pages9
JournalKorean Journal of Chemical Engineering
Volume34
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

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Metabolites
Fructose
Carbon
Acetates
Acetyl Coenzyme A
Boiling liquids
Ethanol
Cells
asoxime chloride

Keywords

  • Adenylate Energy Charge (AEC) Ratio
  • GC-EI-MS
  • LC/TOF/MS
  • Metabolite Profiling
  • PHA
  • Ralstonia eutropha H16

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources. / Im, Dae Kyun; Yun, Seok Hun; Jung, Joon Young; Lee, Jinwon; Oh, Min-Kyu.

In: Korean Journal of Chemical Engineering, Vol. 34, No. 3, 01.03.2017, p. 797-805.

Research output: Contribution to journalArticle

Im, Dae Kyun ; Yun, Seok Hun ; Jung, Joon Young ; Lee, Jinwon ; Oh, Min-Kyu. / Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources. In: Korean Journal of Chemical Engineering. 2017 ; Vol. 34, No. 3. pp. 797-805.
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