Comparison of Photorhabdus luminescens and Vibrio fischeri lux fusions to study gene expression patterns

Robert J. Mitchell, Joo Myung Ahn, Man Bock Gu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A comparison of promoter fusions with the luxCDABE genes from Vibrio fischeri and Photorhabdus luminescens was made using promoters from several genes (katG, sodA, and pqi-5) of E. coli that are responsive to oxidative damage. The respective characteristics, such as the basal and maximum bioluminescence and the relative bioluminescence, were compared. E. coli strains carrying fusions of the promoters to P. luminescens lux showed higher basal and maximally induced bioluminescent levels than strains carrying the same promoter fused to the luxCDABE genes from V. fischeri. The sensitivities between the strains were similar, regardless of the luciferase used, but lower response ratios were seen from strains harboring the P. luminescens lux fusions. Furthermore, using the two katG::lux fusion strains, the bioluminescence from the P. luminescens lux fusion strain, DK1, was stable after reaching a maximum, while that of strain DPD2511 decreased very rapidly due to substrate limitation.

Original languageEnglish
Pages (from-to)48-54
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number1
Publication statusPublished - 2005 Feb 1
Externally publishedYes

Keywords

  • Bioluminescence
  • Gene fusions
  • LuxCDABE
  • Oxidative damage
  • P. luminescens
  • V. fischeri

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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