Comparison of proteomes in various human plasma preparations by two-dimensional gel electrophoresis

Hyun Jung Kim, Mi Ryung Kim, Eun Jung So, Chan Wha Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Serum or plasma can be utilized in a variety of studies targeted toward the discovery of disease biomarkers. In this study, the proteome profiles of plasma samples prepared using various anticoagulants (EDTA, heparin or citrate), were compared with those of serum using two-dimensional electrophoresis (2-DE). Proteins which evidenced different levels in the plasma and serum were screened and identified using ESI-Q-TOF MS/MS. The proteins which became detectable after the removal of fibrinogen from serum were identified as pigment epithelial differentiating factor (four spots), fetuin-like protein, and the hemopexin precursor. In particular, three proteins, pre-serum amyloid P component, plasma glutathione peroxidase precursor, and tetranectin, evidenced increased volume intensity only in the plasma samples prepared with EDTA.

Original languageEnglish
Pages (from-to)619-625
Number of pages7
JournalJournal of Biochemical and Biophysical Methods
Volume70
Issue number4
DOIs
Publication statusPublished - 2007 Jun 10

Fingerprint

Plasma (human)
Electrophoresis, Gel, Two-Dimensional
Proteome
Electrophoresis
Gels
Plasmas
Serum
Edetic Acid
Proteins
Serum Amyloid P-Component
Hemopexin
Fetuins
Protein Precursors
Biomarkers
Glutathione Peroxidase
Pigments
Citric Acid
Anticoagulants
Fibrinogen
Heparin

Keywords

  • 2-DE
  • EDTA
  • ESI-Q-TOF MS/MS
  • Heparin
  • Plasma
  • Serum
  • Sodium citrate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Comparison of proteomes in various human plasma preparations by two-dimensional gel electrophoresis. / Kim, Hyun Jung; Kim, Mi Ryung; So, Eun Jung; Kim, Chan Wha.

In: Journal of Biochemical and Biophysical Methods, Vol. 70, No. 4, 10.06.2007, p. 619-625.

Research output: Contribution to journalArticle

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